Related ArticlesUltrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems. J Vis Exp. 2020 Feb 10;(156): Authors: Takaya T, Iwata K Abstract Femtosecond time-resolved stimulated Raman spectroscopy is a promising method of observing the structural dynamics of short-lived transients with near infrared (near-IR) transitions, because it can overcome the low sensitivity of spontaneous Raman spectrometers in the near-IR...
Tue Feb 25, 2020 15:40
Related ArticlesOaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy. J Vis Exp. 2020 Feb 05;(156): Authors: Deng Y, Zheng B, Liu Y, Shi S, Nie J, Wu T, Zheng P Abstract Chemical and bio-conjugation techniques have been developed rapidly in recent years and allow the building of protein polymers. However, a controlled protein polymerization process is always a challenge. Here, we have...
Tue Feb 25, 2020 15:40
Related ArticlesAsymmetric Thermoelectrochemical Cell for Harvesting Low-grade Heat under Isothermal Operation. J Vis Exp. 2020 Feb 05;(156): Authors: Mu K, Wang X, Ho Li K, Huang YT, Feng SP Abstract Low-grade heat is abundantly available in the environment as waste heat. The efficient conversion of low-grade heat into electricity is very difficult. We developed an asymmetric thermoelectrochemical cell (aTEC) for heat-to-electricity conversion under...
Tue Feb 25, 2020 15:40
Related ArticlesA Step-by-Step Implementation of DeepBehavior, Deep Learning Toolbox for Automated Behavior Analysis. J Vis Exp. 2020 Feb 06;(156): Authors: Shukla S, Arac A Abstract Understanding behavior is the first step to truly understanding neural mechanisms in the brain that drive it. Traditional behavioral analysis methods often do not capture the richness inherent to the natural behavior. Here, we provide detailed step-by-step instructions...
Tue Feb 25, 2020 15:40
Related ArticlesStudying Neurobehavioral Effects of Environmental Pollutants on Zebrafish Larvae. J Vis Exp. 2020 Feb 05;(156): Authors: Zhang B, Yang X, Zhao J, Xu T, Yin D Abstract Recent years more and more environmental pollutants have been proved neurotoxic, especially at the early development stages of organisms. Zebrafish larvae are a preeminent model for the neurobehavioral study of environmental pollutants. Here, a detailed experimental...
Tue Feb 25, 2020 15:40
Related ArticlesSynthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly. J Vis Exp. 2020 Feb 06;(156): Authors: Leguizamon SC, Alqubati AF, Scott TF Abstract This protocol presents the use of Lewis acidic multi-role reagents to circumvent kinetic trapping observed during the self-assembly of information-encoded oligomeric strands mediated by paired dynamic covalent interactions in a manner mimicking the...
Tue Feb 25, 2020 15:40
Related ArticlesFabrication of Nanoheight Channels Incorporating Surface Acoustic Wave Actuation via Lithium Niobate for Acoustic Nanofluidics. J Vis Exp. 2020 Feb 05;(156): Authors: Zhang N, Friend J Abstract Controlled nanoscale manipulation of fluids is known to be exceptionally difficult due to the dominance of surface and viscous forces. Megahertz-order surface acoustic wave (SAW) devices generate tremendous acceleration on their surface, up...
Tue Feb 25, 2020 15:40
Related ArticlesLong-range Channelrhodopsin-assisted Circuit Mapping of Inferior Colliculus Neurons with Blue and Red-shifted Channelrhodopsins. J Vis Exp. 2020 Feb 07;(156): Authors: Goyer D, Roberts MT Abstract When investigating neural circuits, a standard limitation of the in vitro patch clamp approach is that axons from multiple sources are often intermixed, making it difficult to isolate inputs from individual sources with electrical stimulation....
Tue Feb 25, 2020 15:40
Related ArticlesIntegrating Augmented Reality Tools in Breast Cancer Related Lymphedema Prognostication and Diagnosis. J Vis Exp. 2020 Feb 06;(156): Authors: Invernizzi M, Runza L, De Sire A, Lippi L, Blundo C, Gambini D, Boldorini R, Ferrero S, Fusco N Abstract Breast cancer related lymphedema (BCRL) is a detrimental condition characterized by fluid accumulation in the upper limb in breast cancer patients subjected to axillary surgery and/or radiations....
Tue Feb 25, 2020 15:40
Related ArticlesUse of Capillary Electrophoresis Immunoassay to Search for Potential Biomarkers of Amyotrophic Lateral Sclerosis in Human Platelets. J Vis Exp. 2020 Feb 10;(156): Authors: Sage JM, Hall L, McVey A, Barohn RJ, Statland JM, Jawdat O, Dimachkie MM, Agbas A Abstract Capillary electrophoresis immunoassay (CEI), also known as capillary western technology, is becoming a method of choice for screening disease relevant proteins and drugs in...
Tue Feb 25, 2020 15:40
Related ArticlesStereological Estimation of Cholinergic Fiber Length in the Nucleus Basalis of Meynert of the Mouse Brain. J Vis Exp. 2020 Feb 05;(156): Authors: Singh P, Peng DW, Suo WZ Abstract The length of cholinergic or other neuronal axons in various brain regions are often correlated with the specific function of the region. Stereology is a useful method to quantify neuronal profiles of various brain structures. Here we provide a software-based...
Tue Feb 25, 2020 15:40
Related ArticlesAnalyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia. J Vis Exp. 2020 Feb 09;(156): Authors: Mou Y, Mukte S, Chai E, Dein J, Li XJ Abstract Neurons have intense demands for high energy in order to support their functions. Impaired mitochondrial transport along axons has been observed in human neurons, which may contribute to neurodegeneration in various...
Tue Feb 25, 2020 15:40
Related ArticlesGrowth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes. J Vis Exp. 2020 Feb 06;(156): Authors: Walshe J, Abdulsalam NAK, Suzuki S, Chirila TV, Harkin DG Abstract Corneal endothelial cell cultures have a tendency to undergo epithelial-to-mesenchymal transition (EMT) after loss of cell-to-cell contact. EMT is deleterious for the cells as it reduces their ability to form a mature and functional layer. Here,...
Tue Feb 25, 2020 15:40
Related ArticlesQuantification of Tumor Cell Adhesion in Lymph Node Cryosections. J Vis Exp. 2020 Feb 09;(156): Authors: Jandrey EHF, Kuroki MA, Camargo AA, Costa ET Abstract Tumor-draining lymph nodes (LNs) are not merely filters of tumor-produced waste. They are one of the most common regional sites of provisional residence of disseminated tumor cells in patients with different types of cancer. The detection of these LN-residing tumor cells is...
Tue Feb 25, 2020 15:40
Related ArticlesDisplacement Analysis of Myocardial Mechanical Deformation (DIAMOND) Reveals Segmental Heterogeneity of Cardiac Function in Embryonic Zebrafish. J Vis Exp. 2020 Feb 06;(156): Authors: Chen J, Packard RRS Abstract Zebrafish are increasingly utilized as a model organism for cardiomyopathies and regeneration. Current methods evaluating cardiac function fail to reliably detect segmental mechanics and are not readily feasible in zebrafish....
Tue Feb 25, 2020 15:40
Related ArticlesTissue-Engineered Graft for Circumferential Esophageal Reconstruction in Rats. J Vis Exp. 2020 Feb 10;(156): Authors: Kim IG, Wu Y, Park SA, Cho H, Shin JW, Chung EJ Abstract The use of biocompatible materials for circumferential esophageal reconstruction is a technically challenging task in rats and requires an optimal implant technique with nutritional support. Recently, there have been many attempts at esophageal tissue engineering,...
Tue Feb 25, 2020 15:40
Related ArticlesInducing Post-Traumatic Epilepsy in a Mouse Model of Repetitive Diffuse Traumatic Brain Injury. J Vis Exp. 2020 Feb 10;(156): Authors: Shandra O, Robel S Abstract Traumatic brain injury (TBI) is a leading cause of acquired epilepsy. TBI can result in a focal or diffuse brain injury. Focal injury is a result of direct mechanical forces, sometimes penetrating through the cranium, creating a direct lesion in the brain tissue. These are...
Tue Feb 25, 2020 15:40
Related ArticlesLocalization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR. J Vis Exp. 2020 Feb 08;(156): Authors: Ban FX, Yin TY, Guo Q, Pan LL, Liu YQ, Wang XW Abstract Begomoviruses (genus Begomovirus, family Geminiviridae) are transmitted by whiteflies of the Bemisia tabaci complex in a persistent, circulative manner. Considering the extensive damage caused by begomoviruses to crop production...
Tue Feb 25, 2020 15:40
Related ArticlesDextran Labeling and Uptake in Live and Functional Murine Cochlear Hair Cells. J Vis Exp. 2020 Feb 08;(156): Authors: Ballesteros A, Swartz KJ Abstract The hair cell mechanotransduction (MET) channel plays an important role in hearing. However, the molecular identity and structural information of MET remain unknown. Electrophysiological studies of hair cells revealed that the MET channel has a large conductance and is permeable to...
Tue Feb 25, 2020 15:40
Related ArticlesMonitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood. J Vis Exp. 2020 Feb 05;(156): Authors: Naito Y, Osa A, Masuhiro K, Hirai T, Koyama S, Kumanogoh A Abstract Immune checkpoint inhibitors, including PD-1-blocking antibodies, have significantly improved treatment outcomes in various types of cancer. The pharmacological efficacy of these immunotherapies is long lasting, extending even beyond...
Tue Feb 25, 2020 15:40
Related ArticlesMonocular Visual Deprivation and Ocular Dominance Plasticity Measurement in the Mouse Primary Visual Cortex. J Vis Exp. 2020 Feb 08;(156): Authors: Chen K, Zhao Y, Liu T, Su Z, Yu H, Chan LLH, Liu T, Yao D Abstract Monocular visual deprivation is an excellent experimental paradigm to induce primary visual cortical response plasticity. In general, the response of the cortex to the contralateral eye to a stimulus is much stronger than...
Tue Feb 25, 2020 15:40
Related ArticlesCurrent Treatment for Cervical Cancer: An Update. Anticancer Agents Med Chem. 2020 Feb 23;: Authors: Sharma S, Deep A, Sharma AK Abstract Cervical cancer is the towering gynecologic health problem which is considered as the 4th most widespread tumour in women. The prevalence of this fatal ailment is emerging gradually across the globe as about 18.1 million new cancer cases have reported in 2018. The predominance of cervical cancer has...
Tue Feb 25, 2020 15:37
Related ArticlesLong-term topical management of psoriasis: the road ahead. J Dermatolog Treat. 2020 Feb 24;:1-10 Authors: Segaert S, Calzavara-Pinton P, de la Cueva P, Jalili A, Lons Danic D, Pink AE, Thaçi D, Gooderham M Abstract Topical therapies have been available for the treatment of psoriasis for several decades. Despite this and the availability of several types of topicals, with varying potency, and numerous vehicles of administration, the majority...
Tue Feb 25, 2020 15:35
Related ArticlesPrompt gamma spectroscopy for absolute range verification of <sup>12</sup>C ions at synchrotron based facilities. Phys Med Biol. 2020 Feb 24;: Authors: Dal Bello R, Magalhaes Martins P, Brons S, Hermann G, Kihm T, Seimetz M, Seco J Abstract The physical range uncertainty limits the exploitation of the full potential of charged particle therapy. In this work we face this issue aiming to measure the absolute Bragg peak position...
Tue Feb 25, 2020 14:30
Related ArticlesAn evidence-based guideline on the application of molecular testing in the diagnosis, prediction of prognosis, and selection of therapy in non-GIST soft tissue sarcomas. Cancer Treat Rev. 2020 Feb 14;85:101987 Authors: Yao X, Ghert M, Dickson BC, Popovic S, Purgina BM, Verma S, Werier J, Kandel RA Abstract AIMS: To make recommendations on the indications for molecular testing regarding the diagnosis, prediction of prognosis, and treatment...
Tue Feb 25, 2020 14:30
Related ArticlesCurrent applications and challenges of circulating tumor DNA (ctDNA) in squamous cell carcinoma of the head and neck (SCCHN). Cancer Treat Rev. 2020 Feb 14;85:101992 Authors: Galot R, Machiels JH Abstract Liquid biopsies (LB) are emerging in the oncology field, with promising data as new diagnostic, prognostic and treatment-monitoring tools. Squamous cell carcinoma of the head and neck (SCCHN) is a heterogenous disease and many challenges...
Tue Feb 25, 2020 14:30
Related ArticlesPotential application of liposomal nanodevices for non-cancer diseases: an update on design, characterization and biopharmaceutical evaluation. Adv Colloid Interface Sci. 2020 Feb 04;277:102121 Authors: Bayat F, Hosseinpour-Moghadam R, Mehryab F, Fatahi Y, Shakeri N, Dinarvand R, Ten Hagen TLM, Haeri A Abstract Liposomes, lipid-based vesicular systems, have attracted major interest as a means to improve drug delivery to various organs...
Tue Feb 25, 2020 14:30
Related ArticlesExploration of Decisional Control Preferences in Adolescents and Young Adults with Cancer and Other Complex Medical Conditions. J Adolesc Young Adult Oncol. 2020 Feb 24;: Authors: Miano SJ, Douglas SL, Hickman RL, DiMarco M, Piccone C, Daly BJ Abstract Purpose: Adolescent and young adults (AYA) with cancer encounter many medical treatment decisions but may have variable desires for involvement in decision-making. This study describes...
Tue Feb 25, 2020 14:30
Related ArticlesEvaluating Treatment Tolerability in Cancer Clinical Trials using the Toxicity Index. J Natl Cancer Inst. 2020 Feb 24;: Authors: Gresham G, Diniz MA, Razaee ZS, Luu M, Kim S, Hays RD, Piantadosi S, Tighiouart M, Yothers G, Ganz PA, Rogatko A Abstract BACKGROUND: The National Cancer Institute Moonshot℠ research initiative calls for improvements in the analysis and reporting of treatment toxicity to advise key stakeholders on treatment...
Tue Feb 25, 2020 14:30
Related ArticlesLong non-coding RNA DBH-AS1 promotes cancer progression in diffuse large B-cell lymphoma by targeting FN1 via RNA-binding protein BUD13. Cell Biol Int. 2020 Feb 24;: Authors: Song Y, Gao F, Peng Y, Yang X Abstract Diffuse large B-cell lymphoma (DLBC) is a subtype of lymphoma with the worst prognosis. Existing treatment methods are not effective enough due to its high occurrence of metastasis. Therefore, identification of effective therapeutic...
Tue Feb 25, 2020 14:30
Related ArticlesArtificial intelligence in medical imaging: A radiomic guide to precision phenotyping of cardiovascular disease. Cardiovasc Res. 2020 Feb 24;: Authors: Oikonomou EK, Siddique M, Antoniades C Abstract Rapid technological advances in non-invasive imaging, coupled with the availability of large data sets and the expansion of computational models and power, have revolutionized the role of imaging in medicine. Non-invasive imaging is the pillar...
Tue Feb 25, 2020 13:36
Related ArticlesExpanding the Scope of Immunotherapy in Colorectal Cancer: Current Clinical Approaches and Future Directions. Biomed Res Int. 2020;2020:9037217 Authors: Kreidieh M, Mukherji D, Temraz S, Shamseddine A Abstract The success of immune checkpoint inhibitors (ICIs) in an increasing range of heavily mutated tumor types such as melanoma has culminated in their exploration in different subsets of patients with metastatic colorectal cancer (mCRC)....
Tue Feb 25, 2020 13:36
Related ArticlesScreening and Functional Analysis of Hub MicroRNAs Related to Tumor Development in Colon Cancer. Biomed Res Int. 2020;2020:3981931 Authors: Yu DH, Li W, Huang JY, Liu XP, Zhang C, Ruan XL, Li S Abstract Various microRNAs (miRNAs) are of importance in the development of colon cancer, but most of the mechanisms of the miRNAs are still unclear. In order to clarify the hub miRNAs and their roles in colon cancer development, GSE98406 was used...
Tue Feb 25, 2020 13:36
Related ArticlesGap-enhanced Raman tags: fabrication, optical properties, and theranostic applications. Theranostics. 2020;10(5):2067-2094 Authors: Khlebtsov NG, Lin L, Khlebtsov BN, Ye J Abstract Gap-enhanced Raman tags (GERTs) are emerging probes of surface-enhanced Raman scattering (SERS) spectroscopy that have found promising analytical, bioimaging, and theranostic applications. Because of their internal location, Raman reporter molecules are protected...
Tue Feb 25, 2020 13:36
Related ArticlesImaging in Suspected Renal-Cell Carcinoma: Systematic Review. Clin Genitourin Cancer. 2019 04;17(2):e345-e355 Authors: Vogel C, Ziegelmüller B, Ljungberg B, Bensalah K, Bex A, Canfield S, Giles RH, Hora M, Kuczyk MA, Merseburger AS, Powles T, Albiges L, Stewart F, Volpe A, Graser A, Schlemmer M, Yuan C, Lam T, Staehler M Abstract OBJECTIVE: To systematically assessed the diagnostic performance of contrast-enhanced computed tomography...
Tue Feb 25, 2020 13:36
Related ArticlesThe dynamics of hematoma surface regularity and hematoma expansion in acute intracerebral hemorrhage. J Clin Neurosci. 2020 Feb 20;: Authors: Oge DD, Topcuoglu MA, Gocmen R, Arsava EM Abstract The clarification of factors that contribute to hematoma expansion in the setting of intracerebral hemorrhage (ICH) and the relevant physical dynamics are implemental for development of management strategies. Herein, we assessed the interplay between...
Tue Feb 25, 2020 13:35
Related ArticlesEnhancement of behavioral and linguistic outcome measures in autism spectrum disorder through neuro-navigated transcranial magnetic stimulation: A pilot study. J Clin Neurosci. 2020 Feb 20;: Authors: Assadi M PMID: 32089385 [PubMed - as supplied by publisher]
Tue Feb 25, 2020 13:35
Related ArticlesPatterns of management and outcomes of unifocal versus multifocal glioblastoma. J Clin Neurosci. 2020 Feb 20;: Authors: Haque W, Thong Y, Verma V, Rostomily R, Brian Butler E, Teh BS Abstract BACKGROUND: Glioblastoma (GBM) presents as a solitary lesion (unifocal), or as multiple discrete lesions (multifocal). Multifocal GBM may have a worse prognosis as compared to unifocal GBM, but existing data are limited to small institutional series....
Tue Feb 25, 2020 13:35
Publication date: Available online 25 February 2020Source: Annals of Allergy, Asthma & ImmunologyAuthor(s): Alexandria Bozen, Madeleine K. Kanaley, Olivia Negris, Ruchi S. Gupta, Lisa Lombard
Tue Feb 25, 2020 13:29
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