This protocol describes a method for mounting Drosophila larvae to achieve longer than 10 h of uninterrupted time-lapse imaging in intact live animals. This method can be used to image many biological processes close to the larval body wall.
Thu Feb 27, 2020 21:00
This workflow describes the performance of time- and cost-efficient enrichment of multiple protein post-translational modifications (PTMs) simultaneously for quantitative global proteomic analysis. The protocol utilizes peptide-level PTM enrichment with multiple conjugated antibodies, followed by data-independent acquisition mass spectrometry analysis to gain biological insights into PTM crosstalk.
Thu Feb 27, 2020 20:00
This protocol describes the cryoAPEX method, in which an APEX2-tagged membrane protein can be localized by transmission electron microscopy within optimally-preserved cell ultrastructure.
Thu Feb 27, 2020 20:00
This protocol describes the steps taken to induce KRAS lung tumors in mice as well as the quantification of formed tumors by ultrasound imaging. Small tumors are visualized in early timepoints as B-lines. At later timepoints, relative tumor volume measurements are achieved by the measurement tool in the ultrasound software.
Thu Feb 27, 2020 19:00
We describe techniques for differentiation induction of two breast epithelial lines, HC11 and EpH4. While both require fetal calf serum, insulin, and prolactin to produce milk proteins, EpH4 cells can fully differentiate into mammospheres in three-dimensional culture. These complementary models are useful for signal transduction studies of differentiation and neoplasia.
Thu Feb 27, 2020 19:00
Abstract The Internet is a key source of health information, yet little is known about resources for low-risk thyroid cancer treatment. We examined the timeliness, content, quality, readability, and reference to the 2015 American Thyroid Association (ATA) guidelines in websites about thyroid cancer treatment. We identified the top 60 websites using Google, Bing, and Yahoo for “thyroid cancer.” Timeliness and content analysis identified updates in the ATA guidelines (n = 6) and...
Thu Feb 27, 2020 02:00
Abstract In this article, the author discusses his experiences with failure in medicine. He also describes how what he has learned influences his practices today as a radiation oncology resident.
Thu Feb 27, 2020 02:00
New Findings LVAD patients are predisposed to hypertension which may increase the risk of stroke. Hypertension may result from markedly elevated levels of sympathetic nerve activity, which occurs through a baroreceptor‐mediated pathway in response to chronic exposure to a non‐physiologic (and reduced) pulse. Cerebral autoregulatory processes appear to be preserved in the absence of a physiologic pulse. Nevertheless, the rate of ischemic/embolic and hemorrhagic stroke is unacceptably high and...
Thu Feb 27, 2020 20:55
New Findings What is the central question of this study? The goal of this study was to compare locomotor muscle metabo and mechanoreceptor expression in heart failure patients and controls. Further, we investigated if relationships existed between the protein expression and cardiopulmonary responses during exercise with locomotor muscle neural afferent feedback inhibition. What is the main finding and its importance? The novel findings were that heart failure patients exhibited greater protein...
Thu Feb 27, 2020 17:04
New Findings Advanced heart failure patients who are implanted with left ventricular assist devices (LVADs) have a unique circulation that is characterised by a reduced or even absent arterial pulse. The remarkable survival of these patients is accompanied by circulatory complications, including stroke, gastro‐intestinal bleeding and right‐heart failure. Understanding the mechanisms related to the complications in LVAD patients will help the patients and also advance our fundamental understanding...
Thu Feb 27, 2020 17:04
New Findings Incidence of right heart failure post left ventricular assist device (LVAD) implantation is improving, as the understanding of LV‐RV physiology improves. Yet, it remains one of major adverse events affecting LVAD patients. Assessment of RV failure in the context of LVAD physiology should consider its unique anatomical and physiological peculiarities which may advance our understanding of RV function in general. Abstract The adaptation of the right ventricular (RV) output...
Thu Feb 27, 2020 17:04
New Findings Using an approach we developed to measure muscle blood flow by indocyanine green dye detected by near infrared spectroscopy, we have found that isocapnic hyperpnoea at rest leads to an increase in respiratory muscle blood flow that is proportional to the respiratory muscle work required. Surprisingly, cycling exercise interferes with respiratory muscle blood flow, especially in COPD, but even in health athletes. Intercostal muscle blood flow during exercise fails to reach flow rates...
Thu Feb 27, 2020 17:04
Thu Feb 27, 2020 02:00
Oncogenesis, Published online: 26 February 2020; doi:10.1038/s41389-020-0213-4Correction: Role of integrin-linked kinase in regulating the protein stability of the MUC1-C oncoprotein in pancreatic cancer cells
Wed Feb 26, 2020 02:00
ObjectiveFirefighters are exposed to a wide variety of carcinogens during the line of duty, including several associated with head and neck cancer. Existing studies assessing head and neck cancer risk with firefighting have predominately included occupational cohorts or registry data, which are limited by inability to adjust for smoking and alcohol consumption—major risk factors for head and neck cancer. Our objective was to assess the risk of head and neck cancer among men with an occupational history...
Thu Feb 27, 2020 19:00
ObjectivesThere is a paucity of longitudinal population-based studies examining whether changes in work factors are associated with concurrent changes in leisure time physical activity (LTPA). This study examines this issue using 12 years of longitudinal survey data.MethodsData were drawn from the Canadian National Population Health Survey. The initial analytical sample in 1994 of 6407 working individuals was followed every 2 years from 2000 to 2010. Seven work factors were measured as independent...
Thu Feb 27, 2020 19:00
Over the past three millennia, selective breeding has dramatically widened the array of plant domestication traits. However, a close look at the archaeobotanical record illustrates a similar suite of linked traits emerging before humans began selectively breeding food crops. A researchers now summarizes all of these early evolutionary responses in plants, arguing that these shared traits evolved in response to human seed-dispersal services.
Thu Feb 27, 2020 18:44
Scientists have shown that some key points of animal evolution -- like the ones leading to humans or insects -- were associated with a large loss of genes in the genome. The study compared over 100 genomes to investigate what happened at the gene level during the evolution of animals after their origin.
Thu Feb 27, 2020 16:12
Publication date: Available online 27 February 2020Source: Clinical ImagingAuthor(s): Kensaku Mori, Tsukasa Saida, Sodai Hoshiai, Yoko Shibuya, Mana Obata Yasuoka, Toshitaka Ishiguro, Hiroaki Takahashi, Hiromi Hamada, Toyomi Sato, Manabu Minami
Thu Feb 27, 2020 20:07
Abstract Introduction Mastocytosis is associated with mast cell (MC) mediator‐related symptoms for which limited therapies are available. Our aim was to assess the efficacy and safety of omalizumab in the treatment of MC mediator‐related symptoms in adult patients with mastocytosis. Methods We included all published studies and case reports by searching MEDLINE, EMBASE, Web of Science and Cochrane up to April 2019. All adult patients diagnosed with mastocytosis according to WHO criteria...
Thu Feb 27, 2020 20:00
This study aimed to determine the relative amounts of Streptococcus mutans (SM) and lactobacilli (LBs) and their relationship with dental caries among a Yemeni adult population.
Thu Feb 27, 2020 02:00
In the bovine placenta, intimate fetomaternal contact is restricted to placentomes. Within the placentomes fetal chorionic villi interdigitate with corresponding maternal caruncular crypts. The trophoblast epi...
Thu Feb 27, 2020 02:00
Sorghum is one of the most recalcitrant species for transformation. Considering the time and effort required for stable transformation in sorghum, establishing a transient system to screen the efficiency and f...
Thu Feb 27, 2020 02:00
Staphylococcus epidermidis is the primary causative agent of infections associated with indwelling biomaterials. Antibiotic susceptibility patterns, Biofilm formation capability, and screening of responsible gene...
Thu Feb 27, 2020 02:00
To analyze a training program in accident prevention and care and Pediatric Basic Cardiopulmonary Resuscitation taught by medical students.
Thu Feb 27, 2020 02:00
Thu Feb 27, 2020 02:00
Thu Feb 27, 2020 02:00
Publication date: Available online 27 February 2020Source: Journal of the American College of SurgeonsAuthor(s): Joaquim M. Havens, Manuel Castillo-Angeles, Molly Jarman, Daniel Sturgeon, Ali Salim, Zara Cooper
Thu Feb 27, 2020 19:48
Publication date: Available online 27 February 2020Source: Journal of the American College of SurgeonsAuthor(s): John W. Kunstman, Whitney S. Brandt, Sara Abou Azar, Raymond A. Jean, Ronald R. Salem
Thu Feb 27, 2020 19:48
Publication date: Available online 27 February 2020Source: Journal of the American College of SurgeonsAuthor(s): Jaime Ruiz-Tovar, Carolina Llavero, Montiel Jimenez-Fuertes, Manuel Duran, Mercedes Perez-Lopez, Andres Garcia-Marin
Thu Feb 27, 2020 19:48
Μια 61χρονη γυναίκα στην Πενσιλβάνια των ΗΠΑ, που βρέθηκε να ουρεί αλκοόλ, το οποίο παράγει ο ίδιος ο οργανισμός της, χωρίς να έχει πιεί στάλα αλκοολούχου ποτού, είναι η πρώτη στον κόσμο που βρέθηκε να έχει το λεγόμενο «σύνδρομο ουρικής ιδιοπαραγωγής αλκοόλ» ή «σύνδρομο αλκοολικής ζύμωσης ουροδόχου κύστης». Η εν λόγω διαταραχή προκαλείται από ένα μικροοργανισμό (μύκητα) στην ουροδόχο κύστη, ο οποίος επιφέρει ζύμωση στα σάκχαρα της ουροδόχου κύστης δημιουργώντας έτσι αλκοόλ, το οποίο μετά ανιχνεύεται...
Thu Feb 27, 2020 17:16
Ένα νέο φάρμακο, που δοκιμάστηκε στο πλαίσιο δύο κλινικών δοκιμών, διαπιστώθηκε ότι μπορεί να μετριάσει τον χρόνιο βήχα και να μειώσει τη συχνότητά του, ακόμη και σε ανθρώπους που βασανίζονται από τέτοιο πρόβλημα για περισσότερα από 15 χρόνια. Εκτιμάται ότι το 4 έως 10% των ενηλίκων παγκοσμίως έχουν ανεξήγητο χρόνιο βήχα, που διαρκεί πάνω από δύο μήνες. Μερικοί άνθρωποι βήχουν χιλιάδες φορές μέσα στην ημέρα για χρόνια, κάτι που τους εξαντλεί, προκαλώντας επίσης και άλλα προβλήματα (κοιλιακοί πόνοι,...
Thu Feb 27, 2020 15:03
Ο κύκλος μίας γυναίκας είναι το αποτέλεσμα μιας πολύπλοκης αλληλεπίδρασης μεταξύ των ωοθηκών, του εγκεφάλου της, των υπολοίπων ενδοκρινών αδένων (όπως ο θυρεοειδής και τα επινεφρίδια) και του λιπώδους ιστού. Ο λιπώδης ιστός του σώματος ρυθμίζει τη κυκλοφορία της λεπτίνης. Η λεπτίνη είναι μία ορμόνη, η οποία ελέγχει την όρεξη, την ενέργεια και επηρεάζει την αναπαραγωγή. Εάν η γυναίκα είναι πολύ αδύνατη, ο εγκέφαλος σταματά τη παραγωγή λεπτίνης. Οι συντονισμένες αλλαγές στα επίπεδα ορμονών προκαλούν...
Thu Feb 27, 2020 13:45
Γράφει η Μίνα Αγγελίνη Ο κορωνοϊός είναι πιο θανατηφόρος από τη γρίπη, σύμφωνα με τα μέχρι τώρα στοιχεία που έχουμε στη διάθεσή μας, όπως προειδοποιεί από το Βερολίνου ο επικεφαλής του γερμανικού Ινστιτούτου Robert Koch (RKI). «Πόσο υψηλό θα είναι το ποσοστό θνησιμότητας του κορωνοϊού, θα φανεί αφού τελειώσει η επιδημία», πρόσθεσε ο Λόταρ Βίλερ, όπως αναφέρει η γερμανική εφημερίδα «Bild». Λίγες ώρες ώρες, ο Γερμανός υπουργός Υγείας, Γενς Σπαν, είχε προειδοποιήσει για τη χώρα του ότι: «Είμαστε...
Thu Feb 27, 2020 13:15
Το γεύμα έχει συνδεθεί κατά κύριο λόγο με την πρόσληψη τροφής ώστε ο οργανισμός να έχει την απαραίτητη ενέργεια για να πραγματοποιήσει βασικές του λειτουργίες καθώς και με το αίσθημα της απόλαυσης. Ωστόσο, πέρα από την κάλυψη της βασικής μας ανάγκης για ενέργεια, η διατροφή φαίνεται ότι δεν είναι απλά κατανάλωση θερμίδων και πρόσληψη ενέργειας. Έχει και μερικές ακόμη διαστάσεις και μας επηρεάζει ποικιλοτρόπως. Το ζήτημα του τρόπου με τον οποίο μας επηρεάζει η διατροφή – πέραν του προφανούς – φαίνεται...
Thu Feb 27, 2020 13:01
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