Related ArticlesImproving Acceptance of Background Noise with Sound Enrichment. J Am Acad Audiol. 2020 Jan 20;:0 Authors: Gordon-Hickey S, Davis S, Lewis L, Van Haneghan J Abstract BACKGROUND: Acceptance of background noise serves as a means to predict likelihood of hearing aid success. Individuals that are able to accept background noise are more likely to be successful with hearing aids. PURPOSE: The aim of the study was to assess the...
Tue Mar 03, 2020 18:08
Related ArticlesA Computerized Functional Skills Assessment and Training Program Targeting Technology Based Everyday Functional Skills. J Vis Exp. 2020 Feb 13;(156): Authors: Harvey PD, Tibiriçá L, Kallestrup P, Czaja SJ Abstract Today, many functional skills are technology-based, so development of a technology-based training program has broad importance. Here we present a computerized functional skills training program that was paired in half of...
Tue Mar 03, 2020 16:06
Related ArticlesRendering SiO2/Si Surfaces Omniphobic by Carving Gas-Entrapping Microtextures Comprising Reentrant and Doubly Reentrant Cavities or Pillars. J Vis Exp. 2020 Feb 11;(156): Authors: Arunachalam S, Domingues EM, Das R, Nauruzbayeva J, Buttner U, Syed A, Mishra H Abstract We present microfabrication protocols for rendering intrinsically wetting materials repellent to liquids (omniphobic) by creating gas-entrapping microtextures (GEMs)...
Tue Mar 03, 2020 16:06
Related ArticlesA Microwave-Assisted Direct Heteroarylation of Ketones Using Transition Metal Catalysis. J Vis Exp. 2020 Feb 16;(156): Authors: Rosen A, Lindsay K, Quillen A, Nguyen Q, Neiser M, Ramirez S, Costan S, Johnson N, Do TD, Ma L Abstract Heteroarylation introduces heteroaryl fragments to organic molecules. Despite the numerous available reactions reported for arylation via transition metal catalysis, the literature on direct heteroarylation...
Tue Mar 03, 2020 16:06
Related ArticlesNucleofection and In Vivo Propagation of Chicken Eimeria Parasites. J Vis Exp. 2020 Feb 14;(156): Authors: Duan C, Tang X, Hu D, Zhang S, Liu J, Bi F, Hao Z, Suo J, Yu Y, Wang M, Sun P, Du L, Suo X, Liu X Abstract Transfection is a technical process through which genetic material, such as DNA and double-stranded RNA, are delivered into cells to modify the gene of interest. Currently, transgenic technology is becoming an indispensable...
Tue Mar 03, 2020 16:06
Related ArticlesTwo Different Real-Time Place Preference Paradigms Using Optogenetics within the Ventral Tegmental Area of the Mouse. J Vis Exp. 2020 Feb 12;(156): Authors: Bimpisidis Z, König N, Wallén-Mackenzie Å Abstract Understanding how neuronal activation leads to specific behavioral output is fundamental for modern neuroscience. Combining optogenetics in rodents with behavioral testing in validated paradigms allows the measurement of behavioral...
Tue Mar 03, 2020 16:06
Related ArticlesAgrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes. J Vis Exp. 2020 Feb 14;(156): Authors: Masters A, Kang M, McCaw M, Zobrist JD, Gordon-Kamm W, Jones T, Wang K Abstract Demonstrated here is a detailed protocol for Agrobacterium-mediated genetic transformation of maize inbred lines using morphogenic genes Baby boom (Bbm) and Wuschel2 (Wus2). Bbm is regulated by the maize...
Tue Mar 03, 2020 16:06
Related ArticlesAssessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage. J Vis Exp. 2020 Feb 15;(156): Authors: Bose B, Kapoor S, Sen U, Nihad As M, Chaudhury D, Shenoy P S Abstract The ocular surface is subjected to regular wear and tear due to various environmental factors. Exposure to UV-C radiation constitutes an occupational health hazard. Here, we demonstrate the exposure...
Tue Mar 03, 2020 16:06
Related ArticlesVirtual Prism Adaptation Therapy: Protocol for Validation in Healthy Adults. J Vis Exp. 2020 Feb 12;(156): Authors: Cho S, Kim WS, Park SH, Park J, Paik NJ Abstract Hemispatial neglect is a common impairment after stroke. It is associated with poor functional and social outcomes. Therefore, an adequate intervention is imperative for the successful management of hemispatial neglect. However, the clinical use of various interventions...
Tue Mar 03, 2020 16:06
Related ArticlesSynthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection. J Vis Exp. 2020 Feb 16;(156): Authors: Lee CN, Chiu LH, Fang CL, Yeh SD, Zuo CS, Chen SC, Kuo LK, Wang YM, Lai WT Abstract A molecular imaging probe comprising superparamagnetic iron oxide (SPIO) nanoparticles and Mycobacterium tuberculosis surface antibody (MtbsAb) was synthesized to enhance imaging sensitivity...
Tue Mar 03, 2020 16:06
Related ArticlesIsolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC). J Vis Exp. 2020 Feb 13;(156): Authors: Alexandersson I, Harms MJ, Boucher J Abstract White adipose tissue (WAT) dysregulation plays a central role in development of insulin resistance and type 2 diabetes (T2D). To develop new treatments for T2D, more physiologically relevant in vitro adipocyte models are required. This study...
Tue Mar 03, 2020 16:06
Related ArticlesInguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets. J Vis Exp. 2020 Feb 16;(156): Authors: Peng Y, Zou Z, Chen J, Zhang H, Lu Y, Bittino R, Fu H, Cooper DKC, Lin S, Cao M, Dai Y, Cai Z, Mou L Abstract Pancreatic islet transplantation is a well-established therapeutic treatment for type 1 diabetes. The kidney capsule is the most commonly used site for islet transplantation in rodent models. However,...
Tue Mar 03, 2020 16:06
Related ArticlesQuantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography. J Vis Exp. 2020 Feb 11;(156): Authors: Lovely CB Abstract Fetal Alcohol Spectrum Disorders (FASD) describe a highly variable continuum of ethanol-induced developmental defects, including facial dysmorphologies and neurological impairments. With a complex pathology, FASD affects approximately 1 in 100 children born in the United States each year....
Tue Mar 03, 2020 16:06
Related ArticlesAnalyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells. J Vis Exp. 2020 Feb 14;(156): Authors: Weber V, Bork K, Horstkorte R, Olzscha H Abstract The human blood-brain barrier (BBB) is characterized by a very low permeability for biomolecules in order to protect and regulate the metabolism of the brain. The BBB is mainly formed out of endothelial cells embedded in collagen...
Tue Mar 03, 2020 16:06
Related ArticlesStable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA. J Vis Exp. 2020 Feb 12;(156): Authors: Taye N, Stanley S, Hubmacher D Abstract Extracellular matrix (ECM) proteins are crucial for skeletal muscle development and homeostasis. The stable knockdown of genes coding for ECM proteins in C2C12 myoblasts can be applied to study the role of these proteins in skeletal...
Tue Mar 03, 2020 16:06
Related ArticlesFabrication of Ti3C2 MXene Microelectrode Arrays for In Vivo Neural Recording. J Vis Exp. 2020 Feb 12;(156): Authors: Driscoll N, Maleski K, Richardson AG, Murphy B, Anasori B, Lucas TH, Gogotsi Y, Vitale F Abstract Implantable microelectrode technologies have been widely used to elucidate neural dynamics at the microscale to gain a deeper understanding of the neural underpinnings of brain disease and injury. As electrodes are miniaturized...
Tue Mar 03, 2020 16:06
Related ArticlesBioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro. J Vis Exp. 2020 Feb 16;(156): Authors: Walters N, Reátegui E Abstract Neutrophil swarming is a cooperative process by which neutrophils seal off a site of infection and promote tissue reorganization. Swarming has classically been studied in vivo in animal models showing characteristic patterns of cell migration. However, in vivo models...
Tue Mar 03, 2020 16:06
Related ArticlesDevelopment of a Larval Zebrafish Infection Model for Clostridioides difficile. J Vis Exp. 2020 Feb 14;(156): Authors: Li J, Ünal CM, Namikawa K, Steinert M, Köster RW Abstract Clostridioides difficile infection (CDI) is considered to be one of the most common healthcare-associated gastrointestinal infections in the United States. The innate immune response against C. difficile has been described, but the exact roles of neutrophils...
Tue Mar 03, 2020 16:06
Related Articles3D Kinematic Analysis for the Functional Evaluation in the Rat Model of Sciatic Nerve Crush Injury. J Vis Exp. 2020 Feb 12;(156): Authors: Wang T, Ito A, Tajino J, Kuroki H, Aoyama T Abstract Compared to the Sciatic Functional Index (SFI), kinematic analysis is a more reliable and sensitive method for performing functional evaluations of sciatic nerve injury rodent models. In this protocol, we describe a novel kinematic analysis method...
Tue Mar 03, 2020 16:06
Related ArticlesAt-Risk Butterfly Captive Propagation Programs to Enhance Life History Knowledge and Effective Ex Situ Conservation Techniques. J Vis Exp. 2020 Feb 11;(156): Authors: Daniels JC, Hill GM, Rossetti KA, Sanchez SJ, Hornfeldt JA Abstract Improving knowledge of ex situ best practices for at-risk butterflies is important for generating successful conservation and recovery program outcomes. Research on such captive populations can also...
Tue Mar 03, 2020 16:06
Related ArticlesA Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals. J Vis Exp. 2020 Feb 14;(156): Authors: Barkley C, Serra R, Peters SB Abstract Tooth innervation allows teeth to sense pressure, temperature and inflammation, all of which are crucial to the use and maintenance of the tooth organ. Without sensory innervation, daily oral activities would cause irreparable damage. Despite its importance, the...
Tue Mar 03, 2020 16:06
Related ArticlesA novel platinum-based chemotherapeutic inducing immunogenic cell death. Oncoimmunology. 2020;9(1):1729022 Authors: Kepp O, Kroemer G PMID: 32117595 [PubMed]
Tue Mar 03, 2020 16:01
Related ArticlesEosinophil accumulation predicts response to melanoma treatment with immune checkpoint inhibitors. Oncoimmunology. 2020;9(1):1727116 Authors: Simon SCS, Hu X, Panten J, Grees M, Renders S, Thomas D, Weber R, Schulze TJ, Utikal J, Umansky V Abstract Eosinophils have been identified as a prognostic marker in immunotherapy of melanoma and suggested to contribute to anti-tumor host defense. However, the influence of immune checkpoint...
Tue Mar 03, 2020 16:01
Related ArticlesT-cells with a single tumor antigen-specific T-cell receptor can be generated in vitro from clinically relevant stem cell sources. Oncoimmunology. 2020;9(1):1727078 Authors: Bonte S, De Munter S, Goetgeluk G, Ingels J, Pille M, Billiet L, Taghon T, Leclercq G, Vandekerckhove B, Kerre T Abstract Chimeric antigen receptor (CAR) T-cells have shown great promise in the treatment of B-cell malignancies. For acute myeloid leukemia (AML),...
Tue Mar 03, 2020 16:01
Related ArticlesMetabolism in the tumor microenvironment: insights from single-cell analysis. Oncoimmunology. 2020;9(1):1726556 Authors: Xiao Z, Locasale JW, Dai Z Abstract The metabolism of both cancer and immune cells in the tumor microenvironment (TME) is poorly understood since most studies have focused on analysis in bulk samples and ex vivo cell culture models. Our recent analyses of single-cell RNA sequencing data suggest that the metabolic...
Tue Mar 03, 2020 16:01
Related ArticlesTumor-targeting oncolytic virus elicits potent immunotherapeutic vaccine responses to tumor antigens. Oncoimmunology. 2020;9(1):1726168 Authors: Luo Y, Lin C, Zou Y, Ju F, Ren W, Lin Y, Wang Y, Huang X, Liu H, Yu Z, Liu P, Tan G, Yuan Q, Zhang J, Huang C, Xia N Abstract Oncolytic viruses represent a promising therapeutic modality, but they have yet to live up to their therapeutic potential. Safety and efficacy concerns impel us to...
Tue Mar 03, 2020 16:01
Related ArticlesS100A4 promotes hepatocellular carcinogenesis by intensifying fibrosis-associated cancer cell stemness. Oncoimmunology. 2020;9(1):1725355 Authors: Li Y, Wang J, Song K, Liu S, Zhang H, Wang F, Ni C, Zhai W, Liang J, Qin Z, Zhang J Abstract A cancer-promoting role of fibrogenesis in the liver has long been speculated; however, the molecular mechanisms regarding this phenomenon are largely unknown. We demonstrated in our previous study...
Tue Mar 03, 2020 16:01
Related ArticlesHelper T cell-dominant tertiary lymphoid structures are associated with disease relapse of advanced colorectal cancer. Oncoimmunology. 2020;9(1):1724763 Authors: Yamaguchi K, Ito M, Ohmura H, Hanamura F, Nakano M, Tsuchihashi K, Nagai S, Ariyama H, Kusaba H, Yamamoto H, Oda Y, Nakamura M, Akashi K, Baba E Abstract Tertiary lymphoid structures (TLSs), clusters of immune cells found around tumor tissue, have been shown to be associated...
Tue Mar 03, 2020 16:01
Related ArticlesOverexpression of microRNA-155 enhances the efficacy of dendritic cell vaccine against breast cancer. Oncoimmunology. 2020;9(1):1724761 Authors: Hodge J, Wang F, Wang J, Liu Q, Saaoud F, Wang Y, Singh UP, Chen H, Luo M, Ai W, Fan D Abstract MicroRNA 155 (miR-155) plays important roles in the regulation of the development and functions of a variety of immune cells. We previously revealed a vital role of miR-155 in regulating the function...
Tue Mar 03, 2020 16:01
Related ArticlesPotent immunomodulatory effects of an anti-CEA-IL-2 immunocytokine on tumor therapy and effects of stereotactic radiation. Oncoimmunology. 2020;9(1):1724052 Authors: Kujawski M, Sherman M, Hui S, Zuro D, Lee WH, Yazaki P, Sherman A, Szpikowska B, Chea J, Lasiewski D, Poku K, Li H, Colcher D, Wong J, Shively JE Abstract While anti-CEA antibodies have no direct effect on CEA-positive tumors, they can be used to direct potent anti-tumor...
Tue Mar 03, 2020 16:01
Related ArticlesResponse of metastatic mouse invasive lobular carcinoma to mTOR inhibition is partly mediated by the adaptive immune system. Oncoimmunology. 2020;9(1):1724049 Authors: Klarenbeek S, Doornebal CW, Kas SM, Bonzanni N, Bhin J, Braumuller TM, van der Heijden I, Opdam M, Schouten PC, Kersten K, de Bruijn R, Zingg D, Yemelyanenko J, Wessels LFA, de Visser KE, Jonkers J Abstract Effective treatment of invasive lobular carcinoma (ILC) of...
Tue Mar 03, 2020 16:01
Related ArticlesPT-112 induces immunogenic cell death and synergizes with immune checkpoint blockers in mouse tumor models. Oncoimmunology. 2020;9(1):1721810 Authors: Yamazaki T, Buqué A, Ames TD, Galluzzi L Abstract PT-112 is a novel platinum-pyrophosphate conjugate under clinical development for cancer therapy. PT-112 mediates cytostatic and cytotoxic effects against a variety of human and mouse cancer cell lines in vitro. The cytotoxic response...
Tue Mar 03, 2020 16:01
Related ArticlesExpression of TIM3/VISTA checkpoints and the CD68 macrophage-associated marker correlates with anti-PD1/PDL1 resistance: implications of immunogram heterogeneity. Oncoimmunology. 2020;9(1):1708065 Authors: Kato S, Okamura R, Kumaki Y, Ikeda S, Nikanjam M, Eskander R, Goodman A, Lee S, Glenn ST, Dressman D, Papanicolau-Sengos A, Lenzo FL, Morrison C, Kurzrock R Abstract Although immunotherapies have achieved remarkable salutary effects...
Tue Mar 03, 2020 16:01
Related ArticlesResponse to: 'Fentanyl versus remifentanil-based TIVA for pediatric scoliosis repair: does it matter?' by Kars et al. Reg Anesth Pain Med. 2020 Feb 28;: Authors: Adler AC, Chandrakantan A PMID: 32114484 [PubMed - as supplied by publisher]
Tue Mar 03, 2020 13:57
Related ArticlesAnalysis of judicial precedent cases regarding epidural injection in chronic pain management in Republic of Korea. Reg Anesth Pain Med. 2020 Feb 28;: Authors: Cho SI, Shin S, Jung H, Moon JY, Lee HJ Abstract BACKGROUND: Although there is a low incidence of complications associated with epidural injections, pain physicians should still remain vigilant for potentially serious adverse outcomes. This study aimed to identify and describe...
Tue Mar 03, 2020 13:57
Related ArticlesResponse to: 'Posterior quadratus lumborum block in the combat environment' by Dhanjal et al. Reg Anesth Pain Med. 2020 Feb 28;: Authors: Burch R, Miller M PMID: 32114482 [PubMed - as supplied by publisher]
Tue Mar 03, 2020 13:57
Publication date: Available online 2 March 2020Source: TalantaAuthor(s): Lu Yang, Bo Hu, Ai-Hua Liu, Yang Zhang
Tue Mar 03, 2020 13:56
Publication date: Available online 2 March 2020Source: TalantaAuthor(s): Jinlin Liu, Da Chen, Peng Wang, Ge Song, Xiaojun Zhang, Zhongli Li, Yanyan Wang, Jingjing Wang, Jun Yang
Tue Mar 03, 2020 13:56
Publication date: Available online 2 March 2020Source: TalantaAuthor(s): Lanlan Xu, Minxing Wu, Lihe Zhao, Hao Han, Siqi Zhang, Pinyi Ma, Ying Sun, Xinghua Wang, Daqian Song
Tue Mar 03, 2020 13:56
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