Described is a proteomics workflow for identifying protein interaction partners from a nuclear subcellular fraction using immunoaffinity enrichment of a given protein of interest and label-free mass spectrometry. The workflow includes subcellular fractionation, immunoprecipitation, filter aided sample preparation, offline cleanup, mass spectrometry, and a downstream bioinformatics pipeline.
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Publication date: Available online 16 November 2019Source: American Journal of Infection ControlAuthor(s): Patrick Murphy, Le Kang, Michele Fleming, Connie Atkinson, Rachel Pryor, Kaila Cooper, Emily Godbout, Michael P. Stevens, Michelle Doll, Gonzalo BearmanWe employed an interrupted time series analysis to assess the impact of ultraviolet-C light disinfection at terminal discharge in an oncology unit and a bone marrow transplant unit on the incidence of hospital-acquired infections. The deployment...
Publication date: Available online 16 November 2019Source: American Journal of Infection ControlAuthor(s): Jason P. Burnham, Emily R. Shives, David K. Warren, Jennifer H. Han, Hilary M. Babcock, CDC Prevention Epicenters ProgramTerminal room cleaning is critical in preventing pathogen transmission; however, the optimal cleaning effectiveness assessment modality is still being investigated. We sequentially compared cleanliness assessment agreement between a fluorescent marker and an adenosine triphosphate...
Publication date: Available online 16 November 2019Source: American Journal of Infection ControlAuthor(s): Kristofer Harris, Fozia Steinkuller
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Publication date: Available online 16 November 2019Source: European Journal of Surgical OncologyAuthor(s): D. Moreno-Ramírez, R. Vieira, R. Kaufmann
Publication date: Available online 16 November 2019Source: European Journal of Surgical OncologyAuthor(s): Tanir M. Allweis, Tehillah Menes, Noa Rotbart, Yael Rapson, Hana Cernik, Inna Bokov, Judith Diment, Ada Magen, Orit Golan, Noa Levi-Bendet, Osnat Givon Madhala, Ahuva GrubsteinAbstractBackgroundBreast cancer patients with lymph node (LN) metastases at diagnosis often undergo neoadjuvant therapy (NAT). Identification of a LN which regressed after NAT remains a challenge.ObjectiveTo evaluate marking...
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Inflammation of the pituitary gland can occur in a variety of primary or secondary disorders. Idiopathic granulomatous hypophysitis is a rare inflammatory disease of the pituitary gland that can closely mimic ...
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Natural selection is a major driver for the origins of adaptations and new species. Whether or not the processes driving adaptation and speciation share a molecular basis remains largely unknown. Here, we show that divergence in hormone signalling contributed to the evolution of complex adaptations and intrinsic reproductive isolation in the Australian wildflower Senecio lautus. We provide evidence that differences in the auxin pathway, a hormone required for plant growth and development, has led...
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Analyst, 2019, Accepted ManuscriptDOI: 10.1039/C9AN02058F, PaperJunli Du, Xiaolu Li, Songsong Ruan, yingchun Li, fan ren, yanjun Cao, xiaoqing wang, Yongmin Zhang, shaoping wu, Jianli LiA novel turn on fluorescent probe with barbituric acid as a unique recognition group has been rational designed and facile synthesized for detecting hydrazine. Probe DPT displays a large emission...The content of this RSS Feed (c) The Royal Society of Chemistry
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Abstract Vicia species, commonly known as vetches, include legume plants which nowadays can be found in many countries around the world. Their use to improve soil health and productivity is crucial in management schemes that make sustainable agriculture possible, but they can also play a part in the phytoremediation of polluted environments. Furthermore, they harbor a large community of rhizospheric microorganisms, such as biodegradative bacteria and plant growth–promoting rhizobacteria,...
Abstract The co-culturing of Pleurotus eryngii var. ferulae and Rhodotorula mucilaginosa was confirmed in our previous studies to be an efficient strategy to improve laccase production by submerged fermentation. To determine the possible regulation principles underlying this behaviour, comparative transcriptomic analysis was performed on P. eryngii var. ferulae to investigate the differential expression of genes in co-culture. RNA-seq analysis showed that genes concerning xenobiotic...
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Background. Physical activity is believed to enhance body functions and sense of wellbeing in general population. Objectives. This study aimed to explore physical activity measures; and the association between those measures, and mental wellbeing among older adults in South and Southeast Asia. Methods. A systematic search was made in CINHAL, EMBASE, PubMed, and PsycINFO. Articles published between 2008 and 2018 were selected with participants aged 60 years and above, living at home, community, supported...
Keshan disease (KD) is an endemic cardiomyopathy, which mainly occurs in China. Selenium deficiency is believed to play an important role in the pathogenesis of KD, but the molecular mechanism of selenium-induced damage remains unclear. To identify the key genes involved in selenium-induced damage, we compared the expression profiles of selenium-related genes between patients with KD and normal controls. Total RNA was isolated, amplified, labeled, and hybridized to Agilent human 4 × 44 K whole genome...
The introduction of resin-based cements and an adhesive-bonding system in daily dental practice has given the opportunity to increase the retention of previously conventional cemented restorations and the optimal results in esthetic. This experimental study employed the 3D Digital Image Correlation Method (3D-DIC) for detecting shrinkage strain in four dual cured composite cements. The aim was to visualize measure, analyze, and compare strain fields in four resin-based cements using the 3D-DIC method....
Background. Serine hydroxymethyltransferase 2 (SHMT2) activity ensures that cells have a survival advantage in ischemic conditions and regulates redox homeostasis. In this study, we aimed to investigate the role of SHMT2 after hepatic ischemia-reperfusion (IR), which involves hypoxia, ischemic conditions, and cell apoptosis. Methods. A 70% IR model was established in C57BL/6J mice with or without SHMT2 knockdown. H&E staining, liver weight/body weight, serum alanine aminotransferase (ALT), and...
Nuclear factor erythroid 2-related factor 2 (Nrf2) is involved in tumor drug resistance, but its role in imatinib resistance of chronic myeloid leukemia (CML) remains elusive. We aimed to investigate the effects of Nrf2 on drug sensitivity, thioredoxin reductase (TrxR) expression, reactive oxygen species (ROS) production, and apoptosis induction in imatinib-resistant CML K562/G01 cells and explored their potential mechanisms. Stable K562/G01 cells with knockdown of Nrf2 were established by infection...
Background/Aims. Hepatocellular carcinoma (HCC) is the lethal digestive cancer and the second leading cause of cancer death in men worldwide. Wnt7a, a 39Kd secreted glycoprotein composed of 349 amino acids, was reported to be related to various diseases. However, its role in HCC has not been studied yet. In this study, using gene expression data and clinical information obtained from the Oncomine and KMplot database, we acknowledged that WNT7A was underexpressed in HCC cancer tissue compared with...
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A discussion of changes impacting the hearing industry and audiology care, and how audiologists may consider adapting their processes and practices.
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Abstract Rationale Benzodiazepines, a class of commonly prescribed drugs, were shown to cause cognitive impairments in several domains. However, the effect of benzodiazepines on social cognition is still unclear. Objectives In order to clarify how benzodiazepines administration affects the identification of facial expressions of emotion (FEE), we conducted...
Abstract Rational Morphine is one of the most well-known and potent analgesic agents; however, it can also induce various side effects. Thus, finding drugs and mechanisms which can potentiate the analgesic effects of low doses of morphine will be a good strategy for pain management. Objective The involvement of μ-opioid receptors and ventral tegmental area...
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Authors: Cintli Martínez-Ortiz-de-Montellano, Juan Felipe de Jesús Torres-Acosta, Isabelle Fourquaux, Carlos Alfredo Sandoval-Castro and Hervé Hoste.<br />Parasite Vol. 26 , page 65<br />Published online: 18/11/2019<br /> Keywords: Ultrastructural changes ; Haemonchus contortus ; Polyphenol-rich plants ; Goats.<img src="http://feeds.feedburner.com/~r/edp_parasite/~4/fqv58pejRco" height="1" width="1" alt=""/>
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