• JoVE: Journal of Visualized Experiments
• Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture

  Here, we present a protocol for the reproducible generation of porcine testicular organoids with testis specific tissue architecture using the commercially available microwell culture system.

  JoVE: Journal of Visualized Experiments

  Thu Oct 03, 2019 12:41
• Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

  Here we present a method to select for novel variants of the E. coli biotin-protein ligase BirA that biotinylates a specific target peptide. The protocol describes the construction of a plasmid for the bacterial display of the target peptide, generation of a BirA library, selection and characterization of BirA variants.

  JoVE: Journal of Visualized Experiments

  Thu Oct 03, 2019 12:00
• Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration

  This protocol describes the dissection and culture of cranial neural crest cells from mouse models, primarily for the study of cell migration. We describe the live imaging techniques used and the analysis of speed and cell shape changes.

  JoVE: Journal of Visualized Experiments

  Thu Oct 03, 2019 11:42
• Applications for Open Source Microplate-Compatible Illumination Panels

  Microplate Assistive Pipetting Light Emitter (M.A.P.L.E.) is a computer-driven device that systematically illuminates microtiter wells to provide guidance for the manual preparation of microplates.  M.A.P.L.E. improves the accuracy of microplate preparation while automating data recordkeeping.  In addition, it can assist with examining microplate quality or aid in the detection of errors.

  JoVE: Journal of Visualized Experiments

  Thu Oct 03, 2019 11:42
• Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

  Described are protocols for the highly efficient genome editing of murine hematopoietic stem and progenitor cells (HSPC) by the CRISPR/Cas9 system to rapidly develop mouse model systems with hematopoietic system-specific gene modifications.

  JoVE: Journal of Visualized Experiments

  Thu Oct 03, 2019 11:00
• Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response

  Minimal erythema dose (MED) testing is used to establish dosage schedules for ultraviolet radiation phototherapy. It can assess individual variation in inflammatory response but lacks methodology for achieving reproducible results. Here, we present a precision implementation of MED and demonstrate its ability to capture individual variation in inflammatory response.

  JoVE: Journal of Visualized Experiments

  Thu Oct 03, 2019 10:41
• Implantation of an Isoproterenol Mini-Pump to Induce Heart Failure in Mice

  The chronic administration of isoproterenol via an implanted osmotic pump has been used widely to mimic advanced heart failure in mice. Here, we describe detailed methods in surgical mini-pump implantation for the continuous isoproterenol administration over 3 weeks, as well as, echocardiographic assessment for the successful model creation.

  JoVE: Journal of Visualized Experiments

  Thu Oct 03, 2019 10:41
• Journal of the American Chemical Society: Latest Articles (ACS Publications)
• [ASAP] Antiferroelectric Phase Transition in a Proton-Transfer Salt of Squaric Acid and 2,3-Dimethylpyrazine

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b04473

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Wed Oct 02, 2019 21:00
• [ASAP] Light-Induced Protein Degradation with Photocaged PROTACs

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b06422

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Wed Oct 02, 2019 21:00
• [ASAP] Aggregation-Induced Energy Transfer in Color-Tunable Multiblock Bottlebrush Nanofibers

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b08133

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Wed Oct 02, 2019 21:00
• [ASAP] Origin of the Difference in Reactivity between Ir Catalysts for the Borylation of C–H Bonds

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b08920

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Wed Oct 02, 2019 21:00
• [ASAP] Total Synthesis of Viridin and Viridiol

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b08577

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Wed Oct 02, 2019 21:00
• [ASAP] Three-Dimensional Nucleic-Acid-Based Constitutional Dynamic Networks: Enhancing Diversity through Complexity of the Systems

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b08709

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Wed Oct 02, 2019 21:00
• [ASAP] Intermolecular Radical Addition to Ketoacids Enabled by Boron Activation

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b09099

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Wed Oct 02, 2019 21:00
• [ASAP] Synthesis of Cage-Shaped Aluminum Aryloxides: Efficient Lewis Acid Catalyst for Stereoselective Glycosylation Driven by Flexible Shift of Four- to Five-Coordination

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b08875

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Wed Oct 02, 2019 21:00
• [ASAP] Correction to “Synthesis of Modified Nucleoside Oligophosphates Simplified: Fast, Pure, and Protecting Group Free”

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b10441

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Wed Oct 02, 2019 21:00
• [ASAP] Synthesis and Isolation of Antiaromatic Expanded Azacoronene via Intramolecular Vilsmeier-Type Reaction

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b09260

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Wed Oct 02, 2019 21:00
• [ASAP] Complete Stereoinversion of <sc>l</sc>-Tryptophan by a Fungal Single-Module Nonribosomal Peptide Synthetase

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b08898

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Wed Oct 02, 2019 21:00
• [ASAP] Correction to “Strong Phonon–Phonon Interactions Securing Extraordinary Thermoelectric Ge_{1–<italic toggle="yes">x</italic>}Sb_{<italic toggle="yes">x</italic>}Te with Zn-Alloying-Induced Band Alignment”

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b10240

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Wed Oct 02, 2019 21:00
• Medgadget
• Printing Custom Flexible Electronics Directly Onto Skin, Bandages, Medical Devices

  Flexible electronics have the potential to help monitor, and even modulate, a number of physiological parameters. While stick-on heart monitors can be manufactured as one-size-fits-all, in applications such as electronic bandages that can monitor and treat a wound, it’s best to be able to create custom devices that suit each patient’s needs. Currently, making flexible electronics requires harsh processing, including chemical baths, hardeni

  Medgadget

  Thu Oct 03, 2019 11:38
• Laser Activated Gold Nanorods Create Silk Seal for Incisions and Wounds

  Staples and sutures are currently used to seal soft tissues together in clinical practice, but researchers at Arizona State University have come up with an alternative technique that resembles welding. It may end up being used to seal tissues during surgeries and to treat wounds, and in many cases simply to enhance the effectiveness of staples and sutures. Moreover, because the seal can be more continuous, covering the entirety of an incision, it

  Medgadget

  Thu Oct 03, 2019 08:57
• New Imaging Technique Reveals Deep Tumors, Helps Attack Them

  Tumors deep inside the body can be very difficult to spot, track, and study. The brain, being surrounded by a thick skull, is particularly challenging to image using light, so MRI and CT are currently the go-to imaging modalities when looking at deeply seated tissues. Now, researchers at Stanford University are reporting the development of new nanoparticles that can be used to light up and image tumors located well below the surface of th

  Medgadget

  Thu Oct 03, 2019 07:52
• Analytical Chemistry
• [ASAP] Nitrobenzoxadiazole Ether-Based Near-Infrared Fluorescent Probe with Unexpected High Selectivity for H₂S Imaging in Living Cells and Mice

  Analytical ChemistryDOI: 10.1021/acs.analchem.9b03383

  Analytical Chemistry

  Wed Oct 02, 2019 21:00
• [ASAP] Synthetic Cannabinoid Receptor Agonists Detection Using Fluorescence Spectral Fingerprinting

  Analytical ChemistryDOI: 10.1021/acs.analchem.9b03037

  Analytical Chemistry

  Wed Oct 02, 2019 21:00
• [ASAP] Maximizing Tandem Mass Spectrometry Acquisition Rates for Shotgun Proteomics

  Analytical ChemistryDOI: 10.1021/acs.analchem.9b02979

  Analytical Chemistry

  Wed Oct 02, 2019 21:00
• [ASAP] Red–Near-Infrared Fluorescent Probe for Time-Resolved in Vivo Alkaline Phosphatase Detection with the Assistance of a Photoresponsive Nanocontainer

  Analytical ChemistryDOI: 10.1021/acs.analchem.9b03497

  Analytical Chemistry

  Wed Oct 02, 2019 21:00
• [ASAP] Sequential Assessment of Multiple Epigenetic Modifications of Cytosine in Whole Genomic DNA by Surface Plasmon Resonance

  Analytical ChemistryDOI: 10.1021/acs.analchem.9b03423

  Analytical Chemistry

  Wed Oct 02, 2019 21:00
• [ASAP] Generalized Heterodyne Configurations for Photoinduced Force Microscopy

  Analytical ChemistryDOI: 10.1021/acs.analchem.9b03712

  Analytical Chemistry

  Wed Oct 02, 2019 21:00
• [ASAP] Target-Induced Payload Amplification for Spherical Nucleic Acid Enzyme (SNAzyme)-Catalyzed Electrochemiluminescence Detection of Circulating microRNAs

  Analytical ChemistryDOI: 10.1021/acs.analchem.9b03001

  Analytical Chemistry

  Wed Oct 02, 2019 21:00
• [ASAP] Microfluidic Chip-Based Wearable Colorimetric Sensor for Simple and Facile Detection of Sweat Glucose

  Analytical ChemistryDOI: 10.1021/acs.analchem.9b03110

  Analytical Chemistry

  Wed Oct 02, 2019 21:00
• [ASAP] Photo-Scanning Electrochemical Microscopy on the Nanoscale with Through-Tip Illumination

  Analytical ChemistryDOI: 10.1021/acs.analchem.9b03347

  Analytical Chemistry

  Wed Oct 02, 2019 21:00
• [ASAP] Nuclease-Assisted Minor Allele Enrichment Using Overlapping Probes-Assisted Amplification-Refractory Mutation System: An Approach for the Improvement of Amplification-Refractory Mutation System-Polymerase Chain Reaction Specificity in Liquid Biopsies

  Analytical ChemistryDOI: 10.1021/acs.analchem.9b03325

  Analytical Chemistry

  Wed Oct 02, 2019 21:00
• [ASAP] Three-Dimensional Microstructure of e-Fe₂O₃ Crystals in Ancient Chinese Sauce Glaze Porcelain Revealed by Focused Ion Beam Scanning Electron Microscopy

  Analytical ChemistryDOI: 10.1021/acs.analchem.9b03244

  Analytical Chemistry

  Wed Oct 02, 2019 21:00
• [ASAP] Sensitive Detection of Gas-Phase Glyoxal by Electron Attachment Reaction Ionization Mass Spectrometry

  Analytical ChemistryDOI: 10.1021/acs.analchem.9b02029

  Analytical Chemistry

  Wed Oct 02, 2019 21:00
• Facial Plastic
• Contributors

  J. REGAN THOMAS, MD, FACS

  Facial Plastic

  Thu Oct 03, 2019 16:07
• Forthcoming Issues

  Update of Today's Facial Skin Rejuvenation Technology

  Facial Plastic

  Thu Oct 03, 2019 16:07
• Management of Lip Complications

  This article discusses complications that may occur after procedures on the lips, specifically focusing on injectable fillers. Evidence-based guidelines and suggested methods to manage these complications are presented in a systematic format.

  Facial Plastic

  Thu Oct 03, 2019 16:07
• Treatment Protocol for Compromised Nasal Skin

  As the number of patients seeking surgical and nonsurgical rhinoplasty continues to increase, the risk of nasal skin compromise after surgery also has risen. Vascular insult to the nasal skin envelope can lead to permanent disfigurement that is nearly impossible to correct. Tissue loss often requires major reconstruction that yields suboptimal cosmetic results. This article discusses prevention, early recognition, and effective treatment that aim

  Facial Plastic

  Thu Oct 03, 2019 16:07
• Filler-Associated Vision Loss

  Soft tissue fillers continue to gain popularity in addressing volume loss and changes associated with facial aging. The rare but devastating complication from iatrogenic vascular occlusion can result in irreversible vision loss. This article discusses the complications of vision loss associated with fillers and reviews applicable treatment techniques and prevention methods.

  Facial Plastic

  Thu Oct 03, 2019 16:07
• Management of Postsurgical Empty Nose Syndrome

  Empty nose syndrome (ENS) is a controversial condition associated with disruption of nasal airflow caused by excessive loss of turbinate tissue. ENS arises after total or near-total inferior turbinate resection. Patients present with intense fixation on the perception of nasal obstruction. Diagnostic tools to assess for empty nose syndrome include a validated patient questionnaire and the office cotton test. Treatment involves topical moisturizat

  Facial Plastic

  Thu Oct 03, 2019 16:07
• Neck Deformities in Plastic Surgery

  This article provides facial plastic surgeons with the insight to avoid and address common pitfalls in neck procedures. Many aesthetic issues are created from overtreatment or undertreatment of components of the neck. Using the platysma muscle as the divide, ease of access to superficial anatomy leads to overtreatment problems, whereas difficulty of access to deeper structures leads to undertreatment problems and to overall imbalances. Strategies

  Facial Plastic

  Thu Oct 03, 2019 16:07
• Contents

  J. Regan Thomas

  Facial Plastic

  Thu Oct 03, 2019 16:07
• Copyright

  ELSEVIER

  Facial Plastic

  Thu Oct 03, 2019 16:07
• Revision Facial Plastic Surgery: Correcting Bad Results

  FACIAL PLASTIC SURGERY CLINICS OF NORTH AMERICA

  Facial Plastic

  Thu Oct 03, 2019 16:07
• Revision Facial Plastic Surgery: Correcting Bad Results

  All facial plastic surgery procedures and treatment modalities have potentially unintended outcomes, possible poor results, or complications. These issues should be minimized through proper preoperative planning, patient selection, and careful technique. However, even in the experience of well-trained and appropriately careful surgeons, complications can and do occur. Planning for correction of these unexpected and unfortunate situations should b

  Facial Plastic

  Thu Oct 03, 2019 16:07
• Management of Surgical Scars

  An ideal scar is flat, thin, and color matched to the surrounding skin. Incision planning, skin closure, and postoperative care are vital to create an inconspicuous scar. Depressed, hypertrophic, and keloid scars each pose unique challenges to the facial plastic surgeon. Several surgical and nonsurgical options exist in the treatment of scars. Appropriate treatment is based on scar location, quality, and size as well as patient history, preferenc

  Facial Plastic

  Thu Oct 03, 2019 16:07
• Tips to Avoid Complications Following Mohs Reconstruction

  To avoid complications or bad results following reconstruction of skin cancer defects, there are several factors that can be incorporated into the design and execution of repairs. These techniques not only decrease the need to correct bad results, they improve the final aesthetic and functional outcome. So although one surgeon might focus on standard corrective treatments, another might use elements of flap design and implementation that will dec

  Facial Plastic

  Thu Oct 03, 2019 16:07
• JAMA Facial Plastic Surgery Online First
• Extended Butterfly Graft for Functional and Cosmetic Correction of Saddle Nose Deformity

  This case report describes the use of an extended butterfly graft for functional and cosmetic correction of saddle nose deformity.

  JAMA Facial Plastic Surgery Online First

  Wed Oct 02, 2019 17:00
• British Association of Plastic Surgeons
• Suture techniques and cartilage grafts in nasal tip surgery: An algorithm in primary and secondary rhinoplasty

  Publication date: Available online 2 October 2019Source: Journal of Plastic, Reconstructive & Aesthetic SurgeryAuthor(s): Mathias Tremp, Sebastian Haack, Barbara Mijuskovic, Martin HaugAbstractObjectiveIn most primary and secondary rhinoplasties, adjustment of the nasal tip in terms of position, projection, and configuration is mandatory. Usually, this is one of the most challenging parts of the operation. The aim of this study was to present a s

  British Association of Plastic Surgeons

  Thu Oct 03, 2019 06:44
• Pre, intra, and/or Post-operative arterial and venous thromboembolism prophylaxis for breast surgery: Systematic review and meta-analysis

  Publication date: Available online 2 October 2019Source: Journal of Plastic, Reconstructive & Aesthetic SurgeryAuthor(s): Kevin M. Klifto, Caresse F. Gurno, Melissa Major, Stella M. Seal, Justin M. Sacks, Gedge D. Rosson, Michele A. ManahanAbstractSummary: Both thromboembolism and excessive bleeding following breast surgery could result in multiple surgical procedures, breast reconstruction failure, or even mortality. This systematic review and m

  British Association of Plastic Surgeons

  Thu Oct 03, 2019 06:44
• Reconstructive Burden and Financial Implications of Wider Excision Margins For Invasive Primary Cutaneous Melanoma

  Publication date: Available online 2 October 2019Source: Journal of Plastic, Reconstructive & Aesthetic SurgeryAuthor(s): Miss. Michelle Chin Lo, Mr. Martin Heaton, Mr. Andrew Snelling, Prof. Marc MoncrieffAbstractBackgroundFor invasive primary cutaneous melanoma, wider excision is advocated to reduce local recurrence risk and improve patient outcomes. Excision detail is controversial, especially in intermediate and high-risk primaries (AJCC pT2-

  British Association of Plastic Surgeons

  Thu Oct 03, 2019 06:44
• Use of a virtual 3D anterolateral thigh model in medical education: Augmentation and not replacement of traditional teaching?

  Publication date: Available online 2 October 2019Source: Journal of Plastic, Reconstructive & Aesthetic SurgeryAuthor(s): Steven Lo, Abdul Shakor Sandel Abaker, Fabio Quondamatteo, Jenny Clancy, Paul Rea, Michael Marriott, Paul ChapmanAbstractThere is a pressing need for simulated forms of medical - and in particular - anatomical learning. Current modalities of teaching are limited to either traditional 2 dimensional forms of learning such as tex

  British Association of Plastic Surgeons

  Thu Oct 03, 2019 06:44
• Quality of life in patients with vascular malformations outside the central nervous system – comparison with the general Norwegian population

  Publication date: Available online 2 October 2019Source: Journal of Plastic, Reconstructive & Aesthetic SurgeryAuthor(s): Sigurd Berger, Rune Andersen, Eric Dorenberg, Tone Meyer, Inanna Weiss, Milada Cvancarova Smaastuen, Leiv Arne RosselandSummaryPurposeThe aim of this study was to assess Health-Related Quality of Life (HRQoL) in a population of patient with vascular malformations outside the central nervous system (CNS), and to compare the res

  British Association of Plastic Surgeons

  Thu Oct 03, 2019 06:44
• Retrospective Cohort-Based Comparison of Intraoperative Liposomal Bupivacaine Versus Bupivacaine for Donor Site Iliac Crest Analgesia during Alveolar Bone Grafting

  Publication date: Available online 2 October 2019Source: Journal of Plastic, Reconstructive & Aesthetic SurgeryAuthor(s): Ronak A. Patel, Eric M. Jablonka, Kristine C. Rustad, Brian C. Pridgen, Sarah S. Sorice-Virk, Mimi R. Borrelli, Rohit K. Khosla, H. Peter Lorenz, Arash Momeni, Derrick C. WanAbstractIntroductionBone grafting of alveolar clefts is routinely performed with cancellous bone harvested from the iliac crest. Graft site morbidity is f

  British Association of Plastic Surgeons

  Thu Oct 03, 2019 06:44
• Influence of socio-economic factors on litigation in surgery: addressing the gap in malpractice literature

  Publication date: Available online 2 October 2019Source: Journal of Plastic, Reconstructive & Aesthetic SurgeryAuthor(s): L Kearney, E Concannon, C Rolle, M Hynes, V Malesevic, JL KellySummaryThe practice of medicine is occasionally volatile and increasingly litigious. Within the specialties, Plastic surgery is high-risk with negative outcomes seen as dissatisfaction, as opposed to actual physical harm. To date, most research has focused on poten

  British Association of Plastic Surgeons

  Thu Oct 03, 2019 06:44
• Royal Society of Chemistry (Great Britain) Society for Analytical Chemistry Society of Public Analysts (Great Britain)
• Co-functionalization with phosphate and carboxylic acid on polydiacetylene for colorimetric detection of calcium ion in serum

  Analyst, 2019, Accepted ManuscriptDOI: 10.1039/C9AN00855A, PaperJinyoung Oh, Min Sik Eom, Min Su HanIn this study, co-functionalization with phosphate and carboxylic acid on polydiacetylene (PDA) was proposed to detect calcium ion in serum, inspired by biologically abundant phosphate-calcium ion and carboxylic acid-calcium ion...The content of this RSS Feed (c) The Royal Society of Chemistry

  Royal Society of Chemistry (Great Britain) Society for Analytical Chemistry Society of Public Analysts (Great Britain)

  Wed Oct 02, 2019 16:00
• Increased Ion Throughput Using Tristate Ion-Gate Multiplexing

  Analyst, 2019, Accepted ManuscriptDOI: 10.1039/C9AN01585J, PaperPearl Kwantwi-Barima, Tobias Reinecke, Brian ClowersFor time dispersive ion mobility experiments detail control over the mechanism of ion beam modulation is necessary to establish optimum performance as this parameter greatly influences the temporal width of...The content of this RSS Feed (c) The Royal Society of Chemistry

  Royal Society of Chemistry (Great Britain) Society for Analytical Chemistry Society of Public Analysts (Great Britain)

  Wed Oct 02, 2019 16:00
• pH-dependent photoluminescence “switch-on” nanosensor composed of silver nanoparticles and nitrogen and sulphur co-doped carbon dots for discriminative detection of biothiols

  Analyst, 2019, Accepted ManuscriptDOI: 10.1039/C9AN01488H, PaperFeng Xiang, Jizhou Li, Zhongde LiuVery close structure and property similarities among biothiols such as glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), present a major challenge to achieve their discrimination detection. In this contribution, a...The content of this RSS Feed (c) The Royal Society of Chemistry

  Royal Society of Chemistry (Great Britain) Society for Analytical Chemistry Society of Public Analysts (Great Britain)

  Wed Oct 02, 2019 16:00