Incorporating N-acetylcysteine and tricalcium phosphate into epoxy resin-based sealer improved its biocompatibility and adhesiveness to radicular dentine

Publication date: Available online 11 October 2019Source: Dental MaterialsAuthor(s): Carlos Henrique Ribeiro Camargo, Laís Carolina Landim Gomes, Monique Costa Moreira França, Tatiane Sampaio Bittencourt, Marcia Carneiro Valera, Samira Esteves Afonso Camargo, Marco Cicero BottinoAbstractObjectiveThis in vitro study was designed to evaluate the biocompatibility, adhesiveness, and antimicrobial activity of epoxy resin-based sealer associated with N-Acetylcysteine (NAC) or beta-tricalcium phosphate...

ScienceDirect Publication: Dental Materials

Sat Oct 12, 2019 17:13

Rutin protects human periodontal ligament stem cells from TNF-α induced damage to osteogenic differentiation through suppressing mTOR signaling pathway in inflammatory environment

Publication date: Available online 11 October 2019Source: Archives of Oral BiologyAuthor(s): Bin Zhao, Wenjing Zhang, Yixuan Xiong, Yunpeng Zhang, Linglu Jia, Xin XuAbstractObjectivesTo investigate whether rutin could protect human periodontal ligament stem cells (hPDLSCs) from TNF-α induced damage to osteogenic differentiation in inflammatory environment and detect the underlying mechanism.Materials and methodshPDLSCs were identified by flow cytometery. TNF-α was used to stimulate hPDLSCs to establish...

ScienceDirect Publication: Archives of Oral Biology

Sat Oct 12, 2019 13:24

CD36 expression on oral squamous cell carcinoma cells correlates with enhanced proliferation and migratory activity

Abstract Objective Recent studies have demonstrated the pro‐tumour role of CD36 in multiple cancer types. However, its role has not been well elucidated in oral squamous cell carcinoma (OSCC). Here, we aimed to evaluate the role of CD36 in proliferation and migration of OSCC cells. Methods Human OSCC cell lines HSC‐2, HSC‐3, HSC‐4, and Ca9‐22 were assessed for proliferation by staining with the cell proliferation marker Ki‐67. We also assessed migration activity, and the expression of cell...

Oral Diseases

Fri Oct 11, 2019 16:27

AICAR‐induced activation of AMPK inhibits the migration of TSCC cells by targeting ZO‐1

Abstract Objective To investigate the effect of AMPK on tight junction (TJ) components, ZO‐1 and F‐actin, and the underlying mechanisms of AMPK in inhibiting migration of tongue squamous cell carcinoma (TSCC) cells. Methods Expression and distribution of mRNA and/or protein was detected by qRT‐PCR, western blot or immunofluorescence. The migration ability was tested by scratch and transwell migration assays. Transient knockdown of ZO‐1 was achieved by siRNA transfection. Results AMPK...

Oral Diseases

Fri Oct 11, 2019 11:48

Discrimination of oral squamous cell carcinoma from oral lichen planus by salivary metabolomics

Abstract Objective This study was conducted to distinguish salivary metabolites in oral squamous cell carcinoma (OSCC) from those in oral lichen planus (OLP) to identify practical biomarkers for the discrimination of OSCC from OLP. Subjects and methods: Whole unstimulated saliva samples were collected from patients with OSCC (n = 34) and OLP (n = 26). Hydrophilic metabolites in the saliva samples were comprehensively analysed by capillary electrophoresis mass spectrometry. To evaluate the...

Oral Diseases

Thu Oct 10, 2019 19:19