JoVE: Journal of Visualized Experiments , 

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

The fabrication process of a PDMS-based, multilayer, microfluidic device that allows in vitro transcription and translation (IVTT) reactions to be performed over prolonged periods is described. Furthermore, a comprehensive overview of the hardware and software required to automate and maintain these reactions for prolonged durations is provided.

JoVE: Journal of Visualized Experiments

Sun Oct 06, 2019 19:51