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Κυριακή 6 Οκτωβρίου 2019

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
A native Western blot method for analyzing endogenous interferon regulatory factor 5 dimerization in the CAL-1 plasmacytoid dendritic cell line is described. This protocol can be applied to other cell lines as well.
JoVE: Journal of Visualized Experiments
Sun Oct 06, 2019 22:53
Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
This protocol provides instructions for implementing multiphoton lithography to fabricate three-dimensional arrays of fluorescent fiducial markers embedded in poly(ethylene glycol)-based hydrogels for use as reference-free, traction force microscopy platforms. Using these instructions, measurement of 3D material strain and calculation of cellular tractions is simplified to promote high-throughput traction force measurements.
JoVE: Journal of Visualized Experiments
Sun Oct 06, 2019 21:52
A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons
Here, a step-by-step protocol for the preparation and cultivation of porcine split corneal buttons is presented. As this organo-typically cultivated organ culture model shows cell death rates within 15 days, comparable to human donor corneas, it represents the first model allowing long-term cultivation of non-human corneas without adding toxic dextran.
JoVE: Journal of Visualized Experiments
Sun Oct 06, 2019 21:52
Iron Nanowire Fabrication by Nano-Porous Anodized Aluminum and its Characterization
In this work, we describe a protocol to fabricate iron nanowires, including the formation of the porous alumina membrane that is used as the template, electrodeposition into templates using electrolyte solution, and the release of the nanowires into the solution.
JoVE: Journal of Visualized Experiments
Sun Oct 06, 2019 20:51

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