Macro-Rheology Characterization of Gill Raker Mucus in the Silver Carp, Hypophthalmichthys molitrix.
Related Articles Macro-Rheology Characterization of Gill Raker Mucus in the Silver Carp, Hypophthalmichthys molitrix. J Vis Exp. 2020 Jul 10;(161): Authors: Bulusu KV, Racan S, Plesniak MW Abstract The silver carp, Hypophthalmichthys molitrix, is an invasive planktivorous filter feeder fish that infested the natural waterways of the upper Mississippi River basin due to its highly efficient filter feeding mechanism. The characteristic organs...
Tue Jul 28, 2020 16:30
Related Articles A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice. J Vis Exp. 2020 Jul 09;(161): Authors: Mateos-Hernández L, Rakotobe S, Defaye B, Cabezas-Cruz A, Šimo L Abstract Ticks are obligatory blood feeding parasites at all stages of development (except eggs) and are recognized as vectors of various pathogens. The use of mouse models in tick research is critical for understanding their biology and...
Tue Jul 28, 2020 16:30
Related Articles In Vitro and In Vivo Delivery of Magnetic Nanoparticle Hyperthermia using a Custom-Built Delivery System. J Vis Exp. 2020 Jul 02;(161): Authors: Duval KEA, Petryk JD, Hoopes PJ Abstract Hyperthermia has long been used in the treatment of cancer. Techniques have varied from the intra-tumoral insertion of hot iron rods, to systemically delivered tumor antibody-targeted magnetic nanoparticles, at temperatures from 39 ˚C (fever-level)...
Tue Jul 28, 2020 16:30
Plasmodium falciparum Gametocyte Culture and Mosquito Infection Through Artificial Membrane Feeding.
Related Articles Plasmodium falciparum Gametocyte Culture and Mosquito Infection Through Artificial Membrane Feeding. J Vis Exp. 2020 Jul 03;(161): Authors: Tripathi AK, Mlambo G, Kanatani S, Sinnis P, Dimopoulos G Abstract Malaria remains one of the most important public health problems, causing significant morbidity and mortality. Malaria is a mosquito borne disease transmitted through an infectious bite from the female Anopheles mosquito....
Tue Jul 28, 2020 16:30
Related Articles An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract. J Vis Exp. 2020 Jul 01;(161): Authors: Monasky R, Villa S, Thangamani S Abstract Candida albicans hyphal morphogenesis in the gastrointestinal (GI) tract is tightly controlled by various environmental signals, and plays an important role in the dissemination and pathogenesis of this opportunistic fungal pathogen. However, methods...
Tue Jul 28, 2020 16:30
Related Articles Pharmacological Validation of the Prepulse Inhibition of Startle Response in Larval Zebrafish using a Commercial Automated System and Software. J Vis Exp. 2020 Jul 02;(161): Authors: Banono NS, Esguerra CV Abstract While there is an abundance of commercial and standardized automated systems and software for performing the prepulse inhibition (PPI) assay in rodents, to the best of our knowledge, all PPI assays performed...
Tue Jul 28, 2020 16:30
Related Articles Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development. J Vis Exp. 2020 Jul 01;(161): Authors: Pegoli G, Lucini F, Mozzetta C, Lanzuolo C Abstract Autosomal dominant Emery-Dreifuss muscular dystrophy (EDMD) is caused by mutations in the LMNA gene, which encodes the A-type nuclear lamins, intermediate filament proteins that sustain the nuclear envelope...
Tue Jul 28, 2020 16:30
Related Articles Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans. J Vis Exp. 2020 Jul 03;(161): Authors: Golden NL, Plagens RN, Kim Guisbert KS, Guisbert E Abstract The heat shock response (HSR) is a cellular stress response induced by cytosolic protein misfolding that functions to restore protein folding homeostasis, or proteostasis. Caenorhabditis elegans occupies a unique and powerful...
Tue Jul 28, 2020 16:30
Related Articles Simulating Impacts of Ice Storms on Forest Ecosystems. J Vis Exp. 2020 Jun 30;(160): Authors: Campbell JL, Rustad LE, Driscoll CT, Halm I, Fahey TJ, Fakhraei H, Groffman PM, Hawley GJ, Leuenberger W, Schaberg PG Abstract Ice storms can have profound and lasting effects on the structure and function of forest ecosystems in regions that experience freezing conditions. Current models suggest that the frequency and intensity...
Tue Jul 28, 2020 16:30
Related Articles An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons. J Vis Exp. 2020 Jul 11;(161): Authors: Stuardo N, Moya-Alvarado G, Ramírez C, Schiavo G, Bronfman FC Abstract Recombinant BDNF containing an Avi sequence (BDNFAvi) is produced in HEK293 cells and then cost-effectively purified by affinity chromatography. A reproducible protocol was developed...
Tue Jul 28, 2020 16:30
Related Articles Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice. J Vis Exp. 2020 Jul 02;(161): Authors: Djurisic M Abstract Acute hippocampal slices have enabled generations of neuroscientists to explore synaptic, neuronal, and circuit properties in detail and with high fidelity. Exploration of LTP and LTD mechanisms, single neuron dendritic computation, and experience-dependent changes in circuitry, would not have been...
Tue Jul 28, 2020 16:30
Related Articles Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model. J Vis Exp. 2020 Jul 03;(161): Authors: Yang Y, Chen Q, Zhou S, Gong X, Xu H, Hong Y, Dai Q, Jiang L Abstract Transgenic mouse models are powerful for understanding the critical genes controlling osteoclast differentiation and activity, and for studying mechanisms and pharmaceutical treatments of osteoporosis. Cathepsin K (Ctsk)-Cre mice have been widely...
Tue Jul 28, 2020 16:30
Related Articles Fabrication of Thin Film Silver/Silver Chloride Electrodes with Finely Controlled Single Layer Silver Chloride. J Vis Exp. 2020 Jul 01;(161): Authors: Tjon KCE, Yuan J Abstract This paper aims to present a protocol to form smooth and well-controlled films of silver/silver chloride (Ag/AgCl) with designated coverage on top of thin film silver electrodes. Thin film silver electrodes sized 80 µm x 80 µm and 160 µm x 160 µm...
Tue Jul 28, 2020 16:30
Related Articles Measuring Statistical Learning Across Modalities and Domains in School-Aged Children Via an Online Platform and Neuroimaging Techniques. J Vis Exp. 2020 Jun 30;(160): Authors: Schneider JM, Hu A, Legault J, Qi Z Abstract Statistical learning, a fundamental skill to extract regularities in the environment, is often considered a core supporting mechanism of the first language development. While many studies of statistical...
Tue Jul 28, 2020 16:30
Related Articles Microfluidic Fabrication Techniques for High-Pressure Testing of Microscale Supercritical CO2 Foam Transport in Fractured Unconventional Reservoirs. J Vis Exp. 2020 Jul 02;(161): Authors: Hosseini H, Guo F, Barati Ghahfarokhi R, Aryana SA Abstract Pressure limitations of many microfluidic platforms have been a significant challenge in microfluidic experimental studies of fractured media. As a result, these platforms have...
Tue Jul 28, 2020 16:30
Related Articles A Cost Effective and Adaptable Scratch Migration Assay. J Vis Exp. 2020 Jun 30;(160): Authors: Burr SD, Stewart JA Abstract Cell migration is a key component in both physiological and pathological events. Normal cell migration is required for essential functions such as development and mounting an immune response. When a defect or alteration occurs with the cell migration process, it can have detrimental outcomes (i.e.,...
Tue Jul 28, 2020 16:30
Related Articles Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues. J Vis Exp. 2020 Jul 08;(161): Authors: Liu C, Wang S, Liu M, Bai F, Chen Z, Wang P, Mi J, Wu X Abstract The isolation and culture of primary melanocytes from skin tissues is very important for biological research and has been widely used for clinical applications. Isolating primary melanocytes from skin tissues by the conventional method usually takes...
Tue Jul 28, 2020 16:30
Related Articles Development of an Individual-Tree Basal Area Increment Model using a Linear Mixed-Effects Approach. J Vis Exp. 2020 Jul 03;(161): Authors: Wang W, Bai Y, Jiang C, Meng J Abstract Here, we developed an individual-tree model of 5-year basal area increments based on a dataset including 21898 Picea asperata trees from 779 sample plots located in Xinjiang Province, northwest China. To prevent high correlations among observations...
Tue Jul 28, 2020 16:30
Related Articles 2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications. J Vis Exp. 2020 Jul 10;(161): Authors: Zhang N, Shi S, Yoo B, Yuan X, Li W, Zhang S Abstract Mass spectrometry (MS)-based sequencing approaches have been shown to be useful in direct sequencing RNA without the need for a complementary DNA (cDNA) intermediate. However, such approaches are...
Tue Jul 28, 2020 16:30
Related Articles Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.). J Vis Exp. 2020 Jun 30;(160): Authors: Medina-Lozano I, Bertolín JR, Zufiaurre R, Díaz A Abstract Vitamins, especially vitamin C, are important micronutrients found in fruits and vegetables. Vitamin C is also a major contributor to their antioxidant capacity. Lettuce is one of the...
Tue Jul 28, 2020 16:30
Related Articles Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions. J Vis Exp. 2020 Jul 02;(161): Authors: Burt M, Bhattachaya R, Okafor AE, Musah S Abstract Kidney disease affects more than 10% of the global population and costs billions of dollars in federal expenditures. The most severe forms of kidney disease and eventual end-stage renal failure are often...
Tue Jul 28, 2020 16:30
Related Articles Low-dose and long-term azithromycin significantly decreases Staphylococcus aureus in the microbiome of refractory CRS patients. Int Forum Allergy Rhinol. 2020 Jul 26;: Authors: Renteria AE, Maniakas A, Mfuna LE, Asmar MH, Gonzalez E, Desrosiers M Abstract BACKGROUND: The sinonasal microbiome is believed to play an important role in the pathophysiology of refractory chronic rhinosinusitis (CRS). We evaluated changes in the microbiome...
Tue Jul 28, 2020 15:58
Comprehensive analysis of the expression and prognosis for CDCAs in head and neck squamous cell carcinoma. PLoS One. 2020;15(7):e0236678 Authors: Wu ZH, Fang M, Zhou Y Abstract Head and neck squamous cell carcinoma (HNSCC), a tumor included oral cavity, lips, larynx, oropharynx, and the nasopharynx et al. The cell division cycle-associated (CDCA) protein family (CDCA1-8) critical for normal cell function and cancer cell proliferation. We explored...
Tue Jul 28, 2020 16:39
Related Articles Pharyngolaryngeal herpes zoster: A pediatric case. Pediatr Int. 2020 Jul 26;: Authors: Yagi H, Horita S, Komura M, Sakai Y, Mukosaka F PMID: 32715557 [PubMed - as supplied by publisher]
Tue Jul 28, 2020 16:39
Related Articles Evaluation and validation of Physiological and Operative Severity Score for the enumeration of mortality and morbidity and Portsmouth-POSSUM scores in predicting morbidity and mortality in patients undergoing head and neck cancer surgeries. Head Neck. 2020 Jul 27;: Authors: Karan N, Siddiqui S, Sharma KS, Pantvaidya GH, Divatia JV, Kulkarni AP Abstract BACKGROUND: Identification of risk factors for perioperative complications...
Tue Jul 28, 2020 16:39
Related Articles Infiltrative laryngeal disease in dogs. J Small Anim Pract. 2020 Jul 26;: Authors: Dixon A, Tivers MS, Packham L, Black V Abstract OBJECTIVES: To describe the clinical features and outcome of neoplastic and inflammatory infiltrative laryngeal disease in dogs. MATERIALS AND METHODS: Medical records at a single referral centre were retrospectively reviewed for dogs diagnosed with infiltrative laryngeal disease by CT or...
Tue Jul 28, 2020 16:39
Related Articles Denosumab-induced osteonecrosis of external auditory canal. Auris Nasus Larynx. 2020 Jul 23;: Authors: Takeda T, Ito T, Onishi I, Yokomura M, Kawashima Y, Fujikawa T, Tsutsumi T Abstract Denosumab is the world's first human monoclonal antibody for the treatment of osteoporosis and shares an active pathway with bisphosphonates, strongly suppressing osteoclast activities. This is the first case report describing a possible relationship...
Tue Jul 28, 2020 16:39
Related Articles Cancer cases and deaths attributable to lifestyle risk factors in Chile. BMC Cancer. 2020 Jul 25;20(1):693 Authors: Rezende LFM, Murata E, Giannichi B, Tomita LY, Wagner GA, Sanchez ZM, Celis-Morales C, Ferrari G Abstract BACKGROUND: To identify modifiable risk factors that contribute to cancer holds important public health relevance for setting up prevention strategies. Therefore, the aim of this study was to estimate the proportion...
Tue Jul 28, 2020 16:39
Related Articles Effects of Arytenoid Adduction Suture Position on Voice Production and Quality. Laryngoscope. 2020 Jul 25;: Authors: Pillutla P, Zhang Z, Chhetri DK Abstract OBJECTIVES/HYPOTHESIS: Arytenoid adduction (AA) is performed to treat unilateral vocal fold paralysis with a large posterior glottal gap. However, the voice effects of AA suture position remain unclear. This study aimed to evaluate voice production and quality as a function...
Tue Jul 28, 2020 16:39
Related Articles Shoulder function after neck dissection: Assessment via a shoulder-specific quality-of-life questionnaire and active shoulder abduction. Auris Nasus Larynx. 2020 Jul 21;: Authors: Imai T, Sato Y, Abe J, Kumagai J, Morita S, Saijo S, Yamazaki T, Asada Y, Matsuura K Abstract OBJECTIVE: Neck dissection results in a high probability of postoperative shoulder functional impairment, even when the spinal accessory nerve is preserved....
Tue Jul 28, 2020 16:39
Related Articles The Role of Palliative Radiotherapy in the Treatment of Spinal Bone Metastases from Head and Neck Tumors-A Multicenter Analysis of a Rare Event. Cancers (Basel). 2020 Jul 18;12(7): Authors: Bostel T, Rühle A, Rackwitz T, Mayer A, Klodt T, Oebel L, Förster R, Schlampp I, Wollschläger D, Rief H, Sprave T, Debus J, Grosu AL, Schmidberger H, Akbaba S, Nicolay NH Abstract This retrospective multi-center analysis aimed to assess the...
Tue Jul 28, 2020 16:39
Related Articles X-Ray and CT Scan Based Prediction of Best Fit Tracheostomy Tube-A Pilot Study. Diagnostics (Basel). 2020 Jul 22;10(8): Authors: Corbett M, Hughes I, O'Shea J, Davey MG, Savage J, Hughes J, Wallis F Abstract Tracheostomy is a commonly performed intervention in patients requiring ventilatory support. The insertion of inappropriately sized tracheostomy tubes carries a risk of decannulation, tissue damage, ventilatory difficulties,...
Tue Jul 28, 2020 16:39
Related Articles Ex vivo modeling of the airflow dynamics and two-and three-dimensional biomechanical effects of suture placements for prosthetic laryngoplasty in horses. Am J Vet Res. 2020 Aug;81(8):665-672 Authors: Lynch NP, Jones SA, Bazley-White LG, Wilson ZF, Raffetto J, Pfau T, Cheetham J, Perkins JD Abstract OBJECTIVE: To identify the degree of left arytenoid cartilage (LAC) abduction that allows laryngeal airflow similar to that in galloping...
Tue Jul 28, 2020 16:39
Related Articles Efficient RNP-directed Human Gene Targeting Reveals SPDEF Is Required for IL-13-induced Mucostasis. Am J Respir Cell Mol Biol. 2020 03;62(3):373-381 Authors: Koh KD, Siddiqui S, Cheng D, Bonser LR, Sun DI, Zlock LT, Finkbeiner WE, Woodruff PG, Erle DJ Abstract Primary human bronchial epithelial cell (HBEC) cultures are a useful model for studies of lung health and major airway diseases. However, mechanistic studies have been...
Tue Jul 28, 2020 16:39
Related Articles Cortical-Basal Ganglia-Cerebellar Networks in Unilateral Vocal Fold Paralysis: A Pilot Study. Laryngoscope. 2020 02;130(2):460-464 Authors: Perez PL, Cueva KL, Rosen CA, Young VN, Naunheim ML, Yung KC, Schneider SL, Mizuiri D, Klein DJ, Houde JF, Hinkley LB, Nagarajan SS, Cheung SW Abstract OBJECTIVES/HYPOTHESIS: To evaluate differences in cortical-basal ganglia-cerebellar functional connectivity between treated unilateral...
Tue Jul 28, 2020 16:39
Related Articles Quantifying vocal fold wound-healing biomechanical property changes. Laryngoscope. 2020 02;130(2):454-459 Authors: Dion GR, Guda T, Mukudai S, Bing R, Lavoie JF, Branski RC Abstract OBJECTIVES: Development of novel vocal fold (VF) therapeutics is limited by a lack of standardized, meaningful outcomes. We hypothesize that automated microindentation-based VF biomechanical property mapping matched to histology permits quantitative...
Tue Jul 28, 2020 16:39
Related Articles Arytenoid vertical height discrepancy in predicting outcomes after unilateral vocal cord medialization. Laryngoscope. 2020 02;130(2):418-422 Authors: Wong E, Smith M, Stone DB, Palme CE, Smith MC, Riffat F Abstract OBJECTIVES/HYPOTHESIS: Unilateral vocal fold paralysis is a structural abnormality that often occurs secondary to dysfunction of the recurrent laryngeal nerve and typically presents as a breathy voice. Medialization...
Tue Jul 28, 2020 16:39
Related Articles Oval window perilymph fistula in child with recurrent meningitis and unilateral hearing loss. BMJ Case Rep. 2020 Jul 26;13(7): Authors: Ng MMY, D'Arco F, Chorbachi R, Nash R Abstract A 2-year-old boy presented to Ears, Nose and Throat (ENT) surgeons with unilateral hearing loss. Following a prodrome of upper respiratory tract infection (URTI), he developed two episodes of pneumococcal meningitis in quick succession. This case...
Tue Jul 28, 2020 14:53
Related Articles Congenital anterior skull base encephaloceles: long-term outcomes after transnasal endoscopic reconstruction. World Neurosurg. 2020 Jul 23;: Authors: Ruggiero J, Zocchi J, Gallo S, Pietrobon G, De Bernardi F, Bignami M, Locatelli D, Castelnuovo P Abstract BACKGROUND: Congenital intranasal encephaloceles (ECs) are rare malformations of the paediatric age, complex to diagnose and treat, above all if associated to genetic syndromes...
Tue Jul 28, 2020 14:53
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