Stem Cell,

Development of a rinsing separation method for exosome isolation and comparison to conventional methods

OBJECTIVE: Exosomes contain valuable biomarkers for many diseases. Tragically, standardized isolation methods and subsequent characterization criteria for exosomes remain limited. Therefore, we developed a new exosome isolation method, termed rinsing separation, and compared its advantages and weaknesses relative to the existing ultracentrifugation and ExoQuick precipitation methods. MATERIALS AND METHODS: Rinsing separation utilizes heparin and glutaraldehyde as a fixative to isolate exosomes,...

European Review » Stem Cell

12:18

MiR-26b regulates cartilage differentiation of bone marrow mesenchymal stem cells in rats through the Wnt/β-catenin signaling pathway

OBJECTIVE: To investigate the influence of micro-ribonucleic acid (miR)-26b on the cartilage differentiation of mesenchymal stem cells (MSCs) in rats and its mechanism. This study aims to provide references for the clinical treatment of orthopedic diseases, such as osteoarthritis. MATERIALS AND METHODS: MSCs were isolated from rat bone marrow, followed by identification of their immunological manifestation and multi-lineage differentiation potential. In addition, miR-26b small-interfering RNA (siRNA)...

European Review » Stem Cell

12:17