Effect of sex hormones on extracellular matrix of lamina propria in rat vocal fold
Ji Min Kim PhD Sung‐Chan Shin MD, PhD Gi‐Cheol Park MD, PhD Jin‐Choon Lee MD, PhD Yun Kyung Jeon MD Sang Jeong Ahn MD Susan Thibeault PhD Byung‐Joo Lee MD, PhD
First published: 10 June 2019 https://doi.org/10.1002/lary.28086
Editor's Note: This Manuscript was accepted for publication on May 13, 2019.
Presented at the American Laryngological Association's 2019 Annual Meeting at COSM, Austin, Texas, U.S.A., May 1–5, 2019.
Supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (2016R1D1A3B01015539). The authors have no other funding, financial relationships, or conflicts of interest to disclose.
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Abstract
Objectives
The role of sex hormones for voice changes in men and women is presently unknown. To determine the effect of sex hormone on the vocal fold, changes of the extracellular matrix (ECM) in vocal fold lamina propria were assessed in orchiectomized (ORX) and ovariectomized (OVX) rats.
Methods
Male and female Sprague‐Dawley rats were divided into sham‐operated control male (CON‐ORX), ORX, sham‐operated female (CON‐OVX), and OVX rats. Histological changes and expression of ECM‐related genes in lamina propria of the vocal fold were evaluated at 4 and 12 weeks after surgery.
Results
Testosterone and estradiol levels decreased in the ORX and OVX groups, respectively. ORX groups did not have significant changes compared with CON‐ORX groups. However, the expression of hyaluronic acid (HA) was decreased in the OVX group compared with the CON‐OVX group. The expression of collagen I in OVX was lower than in the CON‐OVX group. Collagen III levels were elevated at 4 weeks in the OVX group, but collagen III levels were diminished at 12 weeks in the OVX group. Expression of elastin in the ECM was less dense in the OVX group compared with controls. The expression MMP‐1 and MMP‐9 showed significantly increase in the OVX group compared to the CON‐OVX group.
Conclusion
No changes of the ECM‐related genes in the vocal fold lamina propria were observed in ORX groups with reduced testosterone. However, changes of several ECM‐related genes were observed in OVX groups with decreased estrogen. These results indicate that the vocal fold is an estrogen‐sensitive target organ and that decreased estrogen, not testosterone, can affect the expression of several ECM‐related molecules of vocal fold.
Level of Evidence
NA Laryngoscope, 2019
Ji Min Kim PhD Sung‐Chan Shin MD, PhD Gi‐Cheol Park MD, PhD Jin‐Choon Lee MD, PhD Yun Kyung Jeon MD Sang Jeong Ahn MD Susan Thibeault PhD Byung‐Joo Lee MD, PhD
First published: 10 June 2019 https://doi.org/10.1002/lary.28086
Editor's Note: This Manuscript was accepted for publication on May 13, 2019.
Presented at the American Laryngological Association's 2019 Annual Meeting at COSM, Austin, Texas, U.S.A., May 1–5, 2019.
Supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (2016R1D1A3B01015539). The authors have no other funding, financial relationships, or conflicts of interest to disclose.
Read the full text
ePDFPDFTOOLS SHARE
Abstract
Objectives
The role of sex hormones for voice changes in men and women is presently unknown. To determine the effect of sex hormone on the vocal fold, changes of the extracellular matrix (ECM) in vocal fold lamina propria were assessed in orchiectomized (ORX) and ovariectomized (OVX) rats.
Methods
Male and female Sprague‐Dawley rats were divided into sham‐operated control male (CON‐ORX), ORX, sham‐operated female (CON‐OVX), and OVX rats. Histological changes and expression of ECM‐related genes in lamina propria of the vocal fold were evaluated at 4 and 12 weeks after surgery.
Results
Testosterone and estradiol levels decreased in the ORX and OVX groups, respectively. ORX groups did not have significant changes compared with CON‐ORX groups. However, the expression of hyaluronic acid (HA) was decreased in the OVX group compared with the CON‐OVX group. The expression of collagen I in OVX was lower than in the CON‐OVX group. Collagen III levels were elevated at 4 weeks in the OVX group, but collagen III levels were diminished at 12 weeks in the OVX group. Expression of elastin in the ECM was less dense in the OVX group compared with controls. The expression MMP‐1 and MMP‐9 showed significantly increase in the OVX group compared to the CON‐OVX group.
Conclusion
No changes of the ECM‐related genes in the vocal fold lamina propria were observed in ORX groups with reduced testosterone. However, changes of several ECM‐related genes were observed in OVX groups with decreased estrogen. These results indicate that the vocal fold is an estrogen‐sensitive target organ and that decreased estrogen, not testosterone, can affect the expression of several ECM‐related molecules of vocal fold.
Level of Evidence
NA Laryngoscope, 2019
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