Effect of perinatal biotin deficiency on auditory pathway of the Wistar-Albino rats
Nevreste Didem Sonbay Yılmaz, Özer Erdem Gür, Nuray Ensari, Erdogan Bulut, Ozlem Tugce Kaya, Serap Sırvancı, show all
Received 26 Jan 2019, Accepted 28 Apr 2019, Published online: 24 May 2019
Download citation https://doi.org/10.1080/00016489.2019.1614665
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Abstract
Aim: Severe biotin deficiency associated with biotinidase enzyme deficiency in newborns is seen as severe neurological problems and hearing loss. However, the effect on the infant of deficiencies in the maternal diet during pregnancy are not clear.
Material and methods: The study included 16 female Wistar albino rats and 4 male Wistar albino rats, that were mated and then the females were separated into 4 groups. At 40 days after the birth, 3 pups were selected from each group, and these 12 pups were evaluated with DPOAE and ABR electrophysiologically and the cochlea was examined ultrastructurally with electron microscopy.
Results: In the DPOAE evaluation, At 8000 and 11,000 Hz, the signal-noise ratios in the B-N and B-B groups were statistically significantly higher (p < .05). In ABR, lengthening of the latency periods was determined in all the waves at both 8 and 16 kHz in the B-B group. When the IPL periods were examined, lengthening in IPL 1–5 was statistically significant in the B-B group only at 8 kHz.
Conclusions: Biotin can be said to have an effect on hearing pathways. However, specifically where on the hearing pathways that biotin is involved has not been clarified.
Chinese abstract
目的：新生儿生物素严重缺乏与生物素酶缺乏有关, 被视为严重的神经问题和听力损失。然而, 孕期母亲饮食不足对婴儿的影响尚不清楚。
材料与方法：将16只雌性大白鼠与4只雄性大白鼠交配, 再将雌性大白鼠分为4组。出生后40天, 每组选取3只幼鼠, 分别用DPOAE和ABR电生理检测12只幼崽, 并用电镜观察耳蜗的超微结构。
结果：在DPOAE检测中, 在8000和11000赫兹时, B-N组和B-B组的信号噪声比在统计学意义上更高（P<.05）。在ABR检测中, B-B组在所有8 kHz和16 kHz波处的潜伏期均被延长。当检查IPL周期时, B-B组IPL 1-5的延长仅在8kHz时具有统计学意义。
结论：生物素对听觉通路有影响。然而, 在听觉通路上, 生物素参与到底发生在哪个部位还不清楚。
Keywords: Biotin, hearing pathway, DPOAE, ABR, electron microscopy
Nevreste Didem Sonbay Yılmaz, Özer Erdem Gür, Nuray Ensari, Erdogan Bulut, Ozlem Tugce Kaya, Serap Sırvancı, show all
Received 26 Jan 2019, Accepted 28 Apr 2019, Published online: 24 May 2019
Download citation https://doi.org/10.1080/00016489.2019.1614665
Select Language▼
Translator disclaimer
Abstract
Aim: Severe biotin deficiency associated with biotinidase enzyme deficiency in newborns is seen as severe neurological problems and hearing loss. However, the effect on the infant of deficiencies in the maternal diet during pregnancy are not clear.
Material and methods: The study included 16 female Wistar albino rats and 4 male Wistar albino rats, that were mated and then the females were separated into 4 groups. At 40 days after the birth, 3 pups were selected from each group, and these 12 pups were evaluated with DPOAE and ABR electrophysiologically and the cochlea was examined ultrastructurally with electron microscopy.
Results: In the DPOAE evaluation, At 8000 and 11,000 Hz, the signal-noise ratios in the B-N and B-B groups were statistically significantly higher (p < .05). In ABR, lengthening of the latency periods was determined in all the waves at both 8 and 16 kHz in the B-B group. When the IPL periods were examined, lengthening in IPL 1–5 was statistically significant in the B-B group only at 8 kHz.
Conclusions: Biotin can be said to have an effect on hearing pathways. However, specifically where on the hearing pathways that biotin is involved has not been clarified.
Chinese abstract
目的：新生儿生物素严重缺乏与生物素酶缺乏有关, 被视为严重的神经问题和听力损失。然而, 孕期母亲饮食不足对婴儿的影响尚不清楚。
材料与方法：将16只雌性大白鼠与4只雄性大白鼠交配, 再将雌性大白鼠分为4组。出生后40天, 每组选取3只幼鼠, 分别用DPOAE和ABR电生理检测12只幼崽, 并用电镜观察耳蜗的超微结构。
结果：在DPOAE检测中, 在8000和11000赫兹时, B-N组和B-B组的信号噪声比在统计学意义上更高（P<.05）。在ABR检测中, B-B组在所有8 kHz和16 kHz波处的潜伏期均被延长。当检查IPL周期时, B-B组IPL 1-5的延长仅在8kHz时具有统计学意义。
结论：生物素对听觉通路有影响。然而, 在听觉通路上, 生物素参与到底发生在哪个部位还不清楚。
Keywords: Biotin, hearing pathway, DPOAE, ABR, electron microscopy
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