Related ArticlesIs contrast stasis after Pipeline embolization device deployment associated with higher aneurysm occlusion rates? World Neurosurg. 2019 Sep 13;: Authors: Vakharia K, Waqas M, Munich SA, Lim J, Gong A, Chin F, Snyder KV, Siddiqui AH, Levy EI Abstract OBJECTIVE: To assess the association of degree of contrast stasis in intracranial aneurysms (IAs) immediately after Pipeline embolization device (PED; Medtronic, Dublin, Ireland) deployment...
Tue Sep 17, 2019 16:52
Related ArticlesMechanical thrombectomy for middle cerebral artery occlusion suspected of ovarian hyperstimulation syndrome. World Neurosurg. 2019 Sep 13;: Authors: Kuwano A, Kubota Y, Nonaka T, Miyao S, Nakamoto H, Kawamata T Abstract BACKGROUND: Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic disorder associated with infertility treatment. The main pathology of OHSS is intravascular dehydration and hyperestrogenemia. In mild cases, abdominal...
Tue Sep 17, 2019 16:52
Related ArticlesNeuro-Behcet's disease mimicking lymphoma of the brain and coexisting with a dural arteriovenous fistula: A case report. World Neurosurg. 2019 Sep 13;: Authors: Ripa V, Kwon CS, Burks JD, Morcos JJ Abstract Diverse clinical manifestations have characterized reports of central nervous system involvement in Behcet's disease. There is a limited number of published case reports of neuro-Behcet's disease mimicking brain tumor. We present a...
Tue Sep 17, 2019 16:52
Related ArticlesCombined surgical and endovascular carotid access for endovascular thrombectomy in acute ischemic stroke. World Neurosurg. 2019 Sep 13;: Authors: Wiesmann M, Kalder J, Reich A, Dekeyzer S, Riabikin A, Mpotsaris A, Nikoubashman O Abstract BACKGROUND: Conventional carotid catheterization is impossible in 2-5% of acute stroke cases. Surgical carotid cutdown may be a necessary bailout strategy to allow for carotid access. We assessed the...
Tue Sep 17, 2019 16:52
Related ArticlesNon-resectable thoracic Rosai-Dorfman disease: a case report and review of the literature. World Neurosurg. 2019 Sep 13;: Authors: Lin CK, Tsai YT Abstract BACKGROUND: Rosai-Dorfman disease (RDD) is a rare disease that can be triggered by either viral or bacterial infection. Several parts of the body can be involved, from the CNS to the pelvic regions had been reported. At present, there is a serious lack of guidelines as to how to treat...
Tue Sep 17, 2019 16:52
Related ArticlesA Survey of Chemoprophylaxis Techniques in Spine Surgery Among American Neurosurgery Training Programs. World Neurosurg. 2019 Sep 13;: Authors: Macki M, Haider SA, Dakroub B, Anand SK, Fakih M, Elmenini J, Suryadevara R, Chang V Abstract BACKGROUND: A paucity of randomized trials have compared prophylactic dose of unfractionated heparin (UFH) versus low-molecular-weight heparin (LMWH) for the prevention of venous thromboembolic events...
Tue Sep 17, 2019 16:52
Related ArticlesVideo-Assisted Bilateral Thoracoscopic Sympathotomy for Palmar Hyperhidrosis. World Neurosurg. 2019 Sep 13;: Authors: ReFaey K, Grewal SS, Segura-Duran I, Thomas M, Wharen RE Abstract In this video, we demonstrate a case of a 21-year-old right-handed male who presented with palmar hyperhidrosis. His symptoms started at the age of 4 and progressively worsened throughout his life. Multiple medical treatments were used without significant...
Tue Sep 17, 2019 16:52
Related ArticlesCirculating MicroRNAs as a Potential Non-invasive Biomarkers of Spontaneous Intracerebral Hemorrhage. World Neurosurg. 2019 Sep 13;: Authors: Gareev I, Yang G, Sun J, Beylerli O, Chen X, Zhang D, Zhao B, Zhang R, Sun Z, Yang Q, Li L, Pavlov V, Safin S, Zhao S Abstract INTRODUCTION: Spontaneous Intracerebral hemorrhage (ICH) is a common and severe neurological disorder and is associated with high rates of mortality and morbidity. It is...
Tue Sep 17, 2019 16:52
Related ArticlesIntravascular Papillary Endothelial Hyperplasia of the Pineal Region: A Case Report and Review of the Literature. World Neurosurg. 2019 Sep 13;: Authors: Retzlaff AA, Arispe K, Cochran EJ, Zwagerman NT Abstract Intravascular papillary endothelial hyperplasia (IPEH) is a benign vascular lesion that is uncommon in the central nervous system. Reports of central nervous system involvement include intraparenchymal, sellar, cerebellar, brainstem,...
Tue Sep 17, 2019 16:52
Related ArticlesThe effects of music therapy in patients undergoing septorhinoplasty surgery under general anesthesia. Braz J Otorhinolaryngol. 2019 Mar 05;: Authors: Gokçek E, Kaydu A Abstract INTRODUCTION: Music has been used for several years as a relaxation method to reduce stress and anxiety. It is a painless, safe, inexpensive and practical nonpharmacologic therapeutic modality, widely used all over the world. OBJECTIVES: We aimed to evaluate...
Tue Sep 17, 2019 14:05
Related ArticlesIs serum bilirubin level a predictor factor in parotid gland carcinoma? Braz J Otorhinolaryngol. 2019 Mar 08;: Authors: Bulğurcu S, İdil M, Çukurova İ Abstract INTRODUCTION: Bilirubin levels have been associated with risk of several malignancies. The association between pretreatment serum bilirubin levels and overall survival of patients with parotid gland carcinoma is unclear. OBJECTIVES: In this study, we assessed the effect...
Tue Sep 17, 2019 14:05
Related ArticlesIdentification of risk factors for residual cholesteatoma in children and adults: a retrospective study on 110 cases of revision surgery. Braz J Otorhinolaryngol. 2018 Dec 31;: Authors: Volgger V, Lindeskog G, Krause E, Schrötzlmair F Abstract INTRODUCTION: Residual disease after cholesteatoma removal is still a challenge for the otorhinolaryngologist. Scheduled "second-look" surgery and, more recently, radiological screenings are used...
Tue Sep 17, 2019 14:05
Related ArticlesTrastuzumab-Coated Nanoparticles Loaded With Docetaxel for Breast Cancer Therapy. Dose Response. 2019 Jul-Sep;17(3):1559325819872583 Authors: Zhang X, Liu J, Li X, Li F, Lee RJ, Sun F, Li Y, Liu Z, Teng L Abstract Docetaxel (DTX) is commonly used for breast cancer treatment. Tween 80 used for DTX dissolution in its clinical formulation causes severe hypersensitivity and other adverse reactions. In this study, trastuzumab (Tmab)-coated...
Tue Sep 17, 2019 14:05
Related ArticlesThe calcium-sensing receptor: A novel target for treatment and prophylaxis of neratinib-induced diarrhea. Pharmacol Res Perspect. 2019 Oct;7(5):e00521 Authors: Lysyy T, Lalani AS, Olek EA, Diala I, Geibel JP Abstract Diarrhea is one of the most commonly reported adverse effect of hemotherapy and targeted cancer therapies, such as tyrosine kinase inhibitors (TKI), which often significantly impact patient quality of life, morbidity, and...
Tue Sep 17, 2019 14:05
Related ArticlesSingle Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events. J Vis Exp. 2019 Aug 27;(150): Authors: Svec KV, Patterson JB, Naim N, Howe AK Abstract Durotaxis is the process by which cells sense and respond to gradients of tension. In order to study this process in vitro, the stiffness of the substrate underlying a cell must be manipulated. While hydrogels with graded stiffness and long-term...
Tue Sep 17, 2019 16:12
Related ArticlesA Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice. J Vis Exp. 2019 Aug 30;(150): Authors: Villarreal D, Pradhan G, Wu CS, Allred CD, Guo S, Sun Y Abstract Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. The islets primarily consist of five types of hormone-secreting cells: α cells...
Tue Sep 17, 2019 16:12
Related ArticlesMurine Model of Metastatic Liver Tumors in the Setting of Ischemia Reperfusion Injury. J Vis Exp. 2019 Aug 30;(150): Authors: Yazdani HO, Tohme S Abstract Liver ischemia and reperfusion (I/R) injury, a common clinical challenge, remains an inevitable pathophysiological process that has been shown to induce multiple tissue and organ damage. Despite recent advances and therapeutic approaches, the overall morbidity has remained unsatisfactory...
Tue Sep 17, 2019 16:12
Related ArticlesLeaf Area Index Estimation Using Three Distinct Methods in Pure Deciduous Stands. J Vis Exp. 2019 Aug 29;(150): Authors: Černý J, Pokorný R, Haninec P, Bednář P Abstract Accurate estimations of leaf area index (LAI), defined as half of the total leaf surface area per unit of horizontal ground surface area, are crucial for describing the vegetation structure in the fields of ecology, forestry, and agriculture. Therefore, procedures of...
Tue Sep 17, 2019 16:12
Related ArticlesQuantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells. J Vis Exp. 2019 Aug 27;(150): Authors: Vallery TK, Steitz JA Abstract Mechanistic insight arrives from careful study and quantification of specific RNAs and proteins. The relative locations of these biomolecules throughout the cell at specific times can be captured with fluorescence in situ hybridization...
Tue Sep 17, 2019 16:12
Related ArticlesCell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry. J Vis Exp. 2019 Sep 01;(151): Authors: Lopez Perez R, Münz F, Kroschke J, Brauer J, Nicolay NH, Huber PE Abstract The presented method or slightly modified versions have been devised to study specific treatment responses and side effects of various anti-cancer treatments as used in clinical oncology. It enables a quantitative and longitudinal...
Tue Sep 17, 2019 16:12
Related ArticlesA Model of Self-limited Acute Lung Injury by Unilateral Intra-bronchial Acid Instillation. J Vis Exp. 2019 Aug 30;(150): Authors: Tavares AH, Colby JK, Levy BD, Abdulnour RE Abstract Selective intra-bronchial instillation of hydrochloric acid (HCl) to the murine left mainstem bronchus causes acute tissue injury with histopathologic findings similar to human acute respiratory distress syndrome (ARDS). The resulting alveolar edema, alveolar-capillary...
Tue Sep 17, 2019 16:12
Related ArticlesStandard Operating Procedure for Lyssavirus Surveillance of the Bat Population in Taiwan. J Vis Exp. 2019 Aug 27;(150): Authors: Hsu WC, Hsu CL, Tu YC, Chang JC, Tsai KR, Lee F, Hu SC Abstract Viruses within the genus Lyssavirus are zoonotic pathogens, and at least seven lyssavirus species are associated with human cases. Because bats are natural reservoirs of most lyssaviruses, a lyssavirus surveillance program of bats has been conducted...
Tue Sep 17, 2019 16:12
Related ArticlesA Direct and Simple Method to Assess Drosophila melanogaster's Viability from Embryo to Adult. J Vis Exp. 2019 Aug 27;(150): Authors: Rockwell AL, Beaver I, Hongay CF Abstract In Drosophila melanogaster, viability assays are used to determine the fitness of certain genetic backgrounds. Allelic variations can result in partial or complete loss of viability at different stages of development. Our lab has developed a method to assess viability...
Tue Sep 17, 2019 16:12
Related ArticlesOptimized Sealing Process and Real-Time Monitoring of Glass-to-Metal Seal Structures. J Vis Exp. 2019 Sep 02;(151): Authors: Fan Z, Hu K, Huang Z, Zhang Y, Yan H Abstract Residual stress is an essential factor to keeping the hermeticity and robustness of a glass-to-metal seal structure. The purpose of this report is to demonstrate a novel protocol to characterize and measure residual stress in a glass-to-metal seal structure without destroying...
Tue Sep 17, 2019 16:12
Related ArticlesMethod for Recording Broadband High Resolution Emission Spectra of Laboratory Lightning Arcs. J Vis Exp. 2019 Aug 27;(150): Authors: Mitchard D, Clark D, Carr D, Stone C, Haddad A Abstract Lightning is one of the most common and destructive forces in nature and has long been studied using spectroscopic techniques, first with traditional camera film methods and then digital camera technology, from which several important characteristics...
Tue Sep 17, 2019 16:12
Related ArticlesIdentifying Mutations by High Resolution Melting in a TILLING Population of Rice. J Vis Exp. 2019 Sep 02;(151): Authors: Li S, Yu YP, Liu SM, Fu HW, Huang JZ, Shu QY, Tan YY Abstract Target Induced Local Lesions In Genomes (TILLING) is a strategy of reverse genetics for the high-throughput screening of induced mutations. However, the TILLING system has less applicability for insertion/deletion (Indel) detection and traditional TILLING...
Tue Sep 17, 2019 16:12
Related ArticlesA Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System. J Vis Exp. 2019 Aug 30;(150): Authors: Daharsh L, Zhang J, Ramer-Tait A, Li Q Abstract Humanized mice (hu-mice) that feature a functional human immune system have fundamentally changed the study of human pathogens and disease. They can be used to model diseases that are otherwise difficult or impossible to study in humans or other animal...
Tue Sep 17, 2019 16:12
Related ArticlesStochastic Noise Application for the Assessment of Medial Vestibular Nucleus Neuron Sensitivity In Vitro. J Vis Exp. 2019 Aug 28;(150): Authors: Stefani SP, Breen PP, Serrador JM, Camp AJ Abstract Galvanic vestibular stimulation (GVS) has been shown to improve balance measures in individuals with balance or vestibular impairments. This is proposed to be due to the stochastic resonance (SR) phenomenon, which is defined as application of...
Tue Sep 17, 2019 16:12
Related ArticlesUse of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure. J Vis Exp. 2019 Aug 27;(150): Authors: Mao H, Wang X, Chen W, Liu F, Wan Y, Hu S, Xing J Abstract Single-fiber recording has been a classical and effective electrophysiological technique over the last few decades because of its specific application for nerve fibers in the central and peripheral...
Tue Sep 17, 2019 16:12
Related ArticlesA Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction. J Vis Exp. 2019 Aug 27;(150): Authors: Zhang C, Cui M, Cui Y, Hettinghouse A, Liu CJ Abstract Drug Affinity Responsive Target Stability (DARTS) is a robust method for detection of novel small molecule protein targets. It can be used to verify known small molecule-protein interactions and to find potential protein targets...
Tue Sep 17, 2019 16:12
Related ArticlesUsing Unidirectional Rotations to Improve Vestibular System Asymmetry in Patients with Vestibular Dysfunction. J Vis Exp. 2019 Aug 30;(150): Authors: Rassaian N, Sadeghi NG, Sabetazad B, McNerney KM, Burkard RF, Sadeghi SG Abstract The vestibular system provides information about head movement and mediates reflexes that contribute to balance control and gaze stabilization during daily activities. Vestibular sensors are located in the...
Tue Sep 17, 2019 16:12
Related ArticlesPost-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation. J Vis Exp. 2019 Aug 28;(150): Authors: Calabrese B, Powers RM, Slepian AJ, Halpain S Abstract Neurons differentiated in two-dimensional culture from human pluripotent stem-cell-derived neural progenitor cells (NPCs) represent a powerful model system to explore disease mechanisms and carry out...
Tue Sep 17, 2019 16:12
Related ArticlesIn Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells. J Vis Exp. 2019 Sep 02;(151): Authors: Alberts N, Mathangasinghe Y, Nillegoda NB Abstract J-domain proteins (JDPs) form the largest and the most diverse co-chaperone family in eukaryotic cells. Recent findings show that specific members of the JDP family could form transient heterocomplexes in eukaryotes to fine-tune substrate...
Tue Sep 17, 2019 16:12
Related ArticlesIn Silico Modeling Method for Computational Aquatic Toxicology of Endocrine Disruptors: A Software-Based Approach Using QSAR Toolbox. J Vis Exp. 2019 Aug 28;(150): Authors: Bohlen ML, Jeon HP, Kim YJ, Sung B Abstract Computational analyses of toxicological processes enables high-throughput screening of chemical substances and prediction of their endpoints in biological systems. In particular, quantitative structure-activity relationship...
Tue Sep 17, 2019 16:12
Related ArticlesVisualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes. J Vis Exp. 2019 Aug 28;(150): Authors: Creighton BA, Ruffins TW, Lorenzo DN Abstract Astrocytes are among the most abundant cell types in the adult brain, where they play key roles in a multiplicity of functions. As a central player in brain homeostasis, astrocytes supply neurons with vital metabolites and buffer extracellular water, ions,...
Tue Sep 17, 2019 16:12
Related ArticlesEx Vivo Method for Assessing the Mouse Reproductive Tract Spontaneous Motility and a MATLAB-based Uterus Motion Tracking Algorithm for Data Analysis. J Vis Exp. 2019 Sep 01;(151): Authors: Liang KL, Bursova JO, Lam F, Chen X, Obukhov AG Abstract Dysmenorrhea, or painful cramping, is the most common symptom associated with menses in females and its severity can hinder women's everyday lives. Here, we present an easy and inexpensive method...
Tue Sep 17, 2019 16:12
Related ArticlesInvestigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells. J Vis Exp. 2019 Sep 01;(151): Authors: Cheng CK, Wong THY, Yung YL, Chan NCN, Ng MHL Abstract The bulk of the human genome (~98%) is comprised of non-coding sequences. Cis-regulatory elements (CREs) are non-coding DNA sequences that contain binding sites for transcriptional regulators to modulate gene...
Tue Sep 17, 2019 16:12
Related ArticlesPreparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins. J Vis Exp. 2019 Aug 31;(150): Authors: Corrêa ACNTF, Pereira PR, Paschoalin VMF Abstract Liposome nanocapsules have been applied for many purposes in the pharmaceutical, cosmetic, and food industries. Attributes of liposomes include their biocompatibility, biodegradability, non-immunogenicity, non-toxicity, and ability to entrap...
Tue Sep 17, 2019 16:12
Related ArticlesAccurate Determination of the Equilibrium Surface Tension Values with Area Perturbation Tests. J Vis Exp. 2019 Aug 30;(150): Authors: Chung J, Boudouris BW, Franses EI Abstract We demonstrate two robust protocols for determining the equilibrium surface tension (EST) values with area perturbation tests. The EST values should be indirectly determined from the dynamic surface tension (DST) values when surface tension (ST) values are at steady-state...
Tue Sep 17, 2019 16:12
Related ArticlesGeneration of Neurospheres from Mixed Primary Hippocampal and Cortical Neurons Isolated from E14-E16 Sprague Dawley Rat Embryo. J Vis Exp. 2019 Aug 31;(150): Authors: Das G, Gupta V, Khan J, Mukherjee D, Ghosh S Abstract Primary neuron culture is an essential technique in the field of neuroscience. To gain deeper mechanistic insights into the brain, it is essential to have a robust in vitro model that can be exploited for various neurobiology...
Tue Sep 17, 2019 16:12
Related ArticlesEvaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization. J Vis Exp. 2019 Aug 29;(150): Authors: Takahashi T, Igawa T Abstract Flowering plants have a unique sexual reproduction system called 'double fertilization', in which each of the sperm cells precisely fuses with an egg cell or a central cell. Thus, two independent fertilization events take place almost simultaneously. The fertilized...
Tue Sep 17, 2019 16:12
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