• ScienceDirect Publication: Talanta
• ‘Artificial peroxidase’ nanozyme – Enzyme based lactate biosensor

  Publication date: Available online 27 September 2019Source: TalantaAuthor(s): Darya V. Vokhmyanina, Ksenia D. Andreeva, Maria A. Komkova, Elena E. Karyakina, Arkady A. KaryakinAbstractIn contrast to bienzyme biosensors, we propose the nanozyme-enzyme based ones substituting the enzyme peroxidase with the more active and stable nanoparticles “artificial peroxidase”. The use of catalytically synthesized Prussian Blue based nanozymes simplifies asse

  ScienceDirect Publication: Talanta

  Fri Sep 27, 2019 04:00
• Nature Communications - current - nature.com science feeds
• Non-enzymatic roles of human RAD51 at stalled replication forks

  Nature Communications, Published online: 27 September 2019; doi:10.1038/s41467-019-12297-0RAD51 has been implicated in replication fork processing and restart in response to replication stress. Here, authors reveal mechanistic aspects of non-enzymatic roles of RAD51 for fork reversal and cooperation with FBH1.

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• Connective tissue fibroblasts from highly regenerative mammals are refractory to ROS-induced cellular senescence

  Nature Communications, Published online: 27 September 2019; doi:10.1038/s41467-019-12398-wIn regenerative animals, how cells respond to injury signals inducing senescence is unclear. Here, the authors show that cells from highly regenerative mammals are resistant to ROS-induced cellular senescence, but non-regenerating species exhibit mitochondrial dysfunction/senescence in response to hydrogen peroxide exposure.

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• Manipulating energy migration within single lanthanide activator for switchable upconversion emissions towards bidirectional photoactivation

  Nature Communications, Published online: 27 September 2019; doi:10.1038/s41467-019-12374-4Orthogonal light based control of biology is of interest, yet the synthesis of materials capable of this is complex. Here, the authors report on the synthesis of simpler upconversion nanoparticles which used cross-relaxation to change emission spectra from red to green light with a change in NIR wavelength.

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• Dynamic changes in epithelial cell morphology control thymic organ size during atrophy and regeneration

  Nature Communications, Published online: 27 September 2019; doi:10.1038/s41467-019-11879-2One aspect of ageing on immunity is attributed to accelerated thymic atrophy, but the underlying mechanism is still lacking. Here the authors show, using conditional reporter mouse models, that both atrophy and regeneration of the thymus are regulated by rate-limiting morphological changes in epithelial stroma, independent of cell death or proliferation.

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• Complementary vibrational spectroscopy

  Nature Communications, Published online: 27 September 2019; doi:10.1038/s41467-019-12442-9Generally infrared and Raman spectroscopic methods are needed to study the symmetric and asymmetric molecular vibrational modes. Here the authors demonstrate complementary vibrational spectroscopy to organic molecules by simultaneously measuring their symmetric and anti-symmetric vibrations with one setup.

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• The CXCL5/CXCR2 axis is sufficient to promote breast cancer colonization during bone metastasis

  Nature Communications, Published online: 27 September 2019; doi:10.1038/s41467-019-12108-6Breast cancer cells often invade the bone tissue and remain dormant until they are induced to colonize the metastatic site. Here the authors develop an ex vivo co-culture system consisting of bone and cancer cells from mice and show that CXCL5 has a role in metastatic colonization in the bone.

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• FIERY1 promotes microRNA accumulation by suppressing rRNA-derived small interfering RNAs in <i>Arabidopsis</i>

  Nature Communications, Published online: 27 September 2019; doi:10.1038/s41467-019-12379-zArabidopsis AGO1 binds both microRNA (miRNA) and endogenous small interfering RNA (siRNA). Here the authors show that 5′−3′ RNA degradation suppresses siRNA biogenesis from ribosomal RNA to prevent overloading of AGO1 with ribosomal siRNA and maintain miRNA-directed post translational gene silencing.

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications

  Nature Communications, Published online: 27 September 2019; doi:10.1038/s41467-019-12301-7Epitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super-resolution imaging and in vivo detection of proteins.

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• Non-enzymatic roles of human RAD51 at stalled replication forks

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• Connective tissue fibroblasts from highly regenerative mammals are refractory to ROS-induced cellular senescence

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• Manipulating energy migration within single lanthanide activator for switchable upconversion emissions towards bidirectional photoactivation

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• Dynamic changes in epithelial cell morphology control thymic organ size during atrophy and regeneration

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• Complementary vibrational spectroscopy

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• The CXCL5/CXCR2 axis is sufficient to promote breast cancer colonization during bone metastasis

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• FIERY1 promotes microRNA accumulation by suppressing rRNA-derived small interfering RNAs in <i>Arabidopsis</i>

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications

  Nature Communications - current - nature.com science feeds

  Thu Sep 26, 2019 17:00
• JoVE: Journal of Visualized Experiments
• Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics

  Extracellular matrix ligands can be patterned onto polyacrylamide hydrogels to enable the culture of human embryonic stem cells in confined colonies on compliant substrates. This method can be combined with traction force microscopy and biochemical assays to examine the interplay between tissue geometry, cell-generated forces, and fate specification.

  JoVE: Journal of Visualized Experiments

  Sat Sep 28, 2019 12:49
• Technical Detail for Robot Assisted Pancreaticoduodenectomy

  The following manuscript details a stepwise approach to the robot-assisted pancreaticoduodenectomy performed at the University of Pittsburgh Medical Center.

  JoVE: Journal of Visualized Experiments

  Sat Sep 28, 2019 11:48
• Niobium Oxide Films Deposited by Reactive Sputtering: Effect of Oxygen Flow Rate

  Here, we present a protocol for niobium oxide films deposition by reactive sputtering with different oxygen flow rates for use as an electron transport layer in perovskite solar cells.

  JoVE: Journal of Visualized Experiments

  Sat Sep 28, 2019 11:48
• Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

  Provided here is a protocol for investigating the interactions between native form, prefibrillar, and mature amyloid fibrils of different peptides and proteins with mitochondria isolated from different tissues and various areas of the brain.

  JoVE: Journal of Visualized Experiments

  Sat Sep 28, 2019 11:48
• Surface Electromyographic Biofeedback as a Rehabilitation Tool for Patients with Global Brachial Plexus Injury Receiving Bionic Reconstruction

  Optimal functional outcomes after bionic reconstruction in patients with global brachial plexus injury depend on a structured rehabilitation protocol. Surface electromyographic guided training may improve the amplitude, separation and consistency of EMG signals, which - after elective amputation of a functionless hand - control and drive a prosthetic hand.

  JoVE: Journal of Visualized Experiments

  Sat Sep 28, 2019 10:48
• A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

  The described pipeline is designed for the segmentation of electron microscopy datasets larger than gigabytes, to extract whole-cell morphologies. Once the cells are reconstructed in 3D, customized software designed around individual needs can be used to perform a qualitative and quantitative analysis directly in 3D, also using virtual reality to overcome view occlusion.

  JoVE: Journal of Visualized Experiments

  Sat Sep 28, 2019 10:48
• Modified Single-Loop Reconstruction for Pancreaticoduodenectomy

  The technique of modified single-loop reconstruction following pancreaticoduodenectomy separates pancreatic secretion from bile, thus reducing the severity of POPF without prolonging the duration of surgery.

  JoVE: Journal of Visualized Experiments

  Sat Sep 28, 2019 09:48
• DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

  Here, we describe a protocol to create discrete and accurate inorganic nanostructures on substrates using DNA origami shapes as guiding templates. The method is demonstrated by creating plasmonic gold bowtie-shaped antennas on a transparent substrate (sapphire).

  JoVE: Journal of Visualized Experiments

  Fri Sep 27, 2019 12:35
• Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

  This protocol introduces a light-spot assay to investigate Drosophila larval phototactic behavior. In this assay, a light spot is generated as light stimulation, and the process of larval light avoidance is recorded by an infrared light-based imaging system.

  JoVE: Journal of Visualized Experiments

  Fri Sep 27, 2019 12:00
• Quantification of Coenzyme A in Cells and Tissues

  This method describes sample preparation from cultured cells and animal tissues, extraction and derivatization of coenzyme A in the samples, followed by high pressure liquid chromatography for purification and quantification of the derivatized coenzyme A by absorbance or fluorescence detection.

  JoVE: Journal of Visualized Experiments

  Fri Sep 27, 2019 11:35
• Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

  This report describes a microfluidic chip-based method to set up a single cell culture experiment in which high-efficiency pairing and microscopic analysis of multiple single cells can be achieved.

  JoVE: Journal of Visualized Experiments

  Fri Sep 27, 2019 11:35
• Applying an eMASS Customization Program as a Research Tool to Evaluate Consumer Benefits

  Presented here is a protocol to examine consumer responses toward mass customization in the context of online retailing. The protocol details the online survey procedure and how to analyze data using structural equation modeling and group differences using latent mean analyses.

  JoVE: Journal of Visualized Experiments

  Fri Sep 27, 2019 10:34
• Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

  Presented here is a simple-to-use, core/shell, three-dimensional bioprinting set-up for one-step fabrication of hollow scaffolds, suitable for tissue engineering of vascular and other tubular structures.

  JoVE: Journal of Visualized Experiments

  Fri Sep 27, 2019 10:34
• Cheek Injection Model for Simultaneous Measurement of Pain and Itch-related Behaviors

  Typically, the mouse neck injection model is used for evaluate pruritogen-induced scratch behaviors. However, the model provides information only on itch, not pain. Here, a cheek injection model is introduced in mice which can be used to simultaneously measure pain and itch-related behaviors.

  JoVE: Journal of Visualized Experiments

  Fri Sep 27, 2019 09:32
• Journal of the American Chemical Society: Latest Articles (ACS Publications)
• [ASAP] Circularly Polarized Luminescence from Achiral Single Crystals of Hybrid Manganese Halides

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b08780

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Thu Sep 26, 2019 21:00
• [ASAP] Bioactive Synthetic-Bioinformatic Natural Product Cyclic Peptides Inspired by Nonribosomal Peptide Synthetase Gene Clusters from the Human Microbiome

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b07317

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Thu Sep 26, 2019 21:00
• [ASAP] Synthesis, Characterization, and Reactivity of Complex Tricyclic Oxonium Ions, Proposed Intermediates in Natural Product Biosynthesis

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b07438

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Thu Sep 26, 2019 21:00
• [ASAP] Mechanical Susceptibility of a Rotaxane

  Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b06960

  Journal of the American Chemical Society: Latest Articles (ACS Publications)

  Thu Sep 26, 2019 21:00