| Molecular Systems Biology Volume 15, Issue 9September 2019 |
Methods
Open Access
Articles
Open Access
Open Access
Open Access
Open AccessAn integrated workflow for crosslinking mass spectrometry
Marta L Mendes, Lutz Fischer, Zhuo A Chen, Marta Barbon, Francis J O'Reilly, Sven H Giese, Michael Bohlke‐Schneider, Adam Belsom, Therese Dau, Colin W Combe, Martin Graham, Markus R Eisele, Wolfgang Baumeister, Christian Speck, Juri Rappsilber
e8994 | First Published: 20 September 2019
A new workflow combining sequential digestion and the search software Xi increases the number of identified crosslinks in a wide range of applications. By detecting dynamic protein interactions, crosslinking is synergistic with other structural approaches and is promising for drug development.
Open AccessParalog dependency indirectly affects the robustness of human cells
Rohan Dandage, Christian R Landry
e8871 | First Published: 24 September 2019
Meta‐analysis of genome‐wide loss of function screens in human cell lines reveals that deleteriousness of heteromeric paralogs can be largely explained by their inter‐dependency and the unique features of paralog gene expression.
Open AccessEndogenous fluctuations of OCT4 and SOX2 bias pluripotent cell fate decisions
Daniel Strebinger, Cédric Deluz, Elias T Friman, Subashika Govindan, Andrea B Alber, David M Suter
e9002 | First Published: 25 September 2019
This study shows that endogenous OCT4 and SOX2 concentrations fluctuate over a 2–3 fold range in mouse embryonic stem (ES) cells, and that these have a large impact on ES cell differentiation efficiency towards mesendoderm and neuroectoderm.
Open Accessp53 pulse modulation differentially regulates target gene promoters to regulate cell fate decisions
Marie D Harton, Woo Seuk Koh, Amie D Bunker, Abhyudai Singh, Eric Batchelor
e8685 | First Published: 26 September 2019
Small‐molecule perturbation of the amplitude, duration, and frequency of p53 oscillations generates distinct target promoter responses. Oscillation characteristics control promoter activation thresholds, a refractory period, and dynamic filtering.
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