The difference in nasal bacterial microbiome diversity between chronic rhinosinusitis patients with polyps and a control population
Weigang Gan MD Fengjuan Yang MM Yiping Tang MM Danmei Zhou MM Di Qing MM Juanjuan Hu MD Shixi Liu MD Feng Liu MD Juan Meng MD
First published: 05 February 2019 https://doi.org/10.1002/alr.22297 Cited by: 2
The first 2 authors, W.G. and F.Y., contributed equally to this work.
Funding sources for the study: National Science Fund of China (81300815 to J.M., and 81570310000 to S.L.), the 12th Five‐Year Science and Technology Support Program (2014BAI07B04 to J.M.); international cooperation project of Sichuan Provincial Science and Technology Department (2018HH0097 to J.M.); Program of Sichuan Provincial Department of Education (18ZB0213 to W.G.); and Youth Innovation Project of Sichuan Provincial Medical Association (Q15025 to W.G.).
Potential conflict of interest: None provided.
The data presented herein were analyzed in part using free online cloud platform Majorbio I‐Sanger (www.i-sanger.com).
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Abstract
Background
Little is known regarding the role of the microbiome of the paranasal sinuses and its contribution to sinus mucosal health and disease. Consequently, we examined the microbiome of chronic rhinosinusitis patients with polyps (CRSwNP) and a control population to provide new insights into the microbiota associated with the pathogenesis of CRSwNP.
Methods
Fifty‐nine CRSwNP patients and 27 controls were enrolled in the study. The bacterial communities of the middle meatus were detected using 16S ribosomal RNA (rRNA)‒targeted Illumina MiSeq sequencing after microbial DNA was extracted from swabs.
Results
Although there was no difference in diversity between the 2 groups, richness was lower in the CRSwNP group than in the control group (p = 0.03). At the phylum level, Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes were predominant in both groups; however, the relative abundance was different, with the proportions of Actinobacteria (predominantly Corynebacterium) and Dolosigranulum being significantly higher in the control group than in the CRSwNP group.
Conclusion
These results support the theory of microbial dysbiosis as the pathogenesis of CRSwNP. The reduction in the proportions of potentially protective bacteria may decrease the overall stability of the sinonasal bacterial community.
Weigang Gan MD Fengjuan Yang MM Yiping Tang MM Danmei Zhou MM Di Qing MM Juanjuan Hu MD Shixi Liu MD Feng Liu MD Juan Meng MD
First published: 05 February 2019 https://doi.org/10.1002/alr.22297 Cited by: 2
The first 2 authors, W.G. and F.Y., contributed equally to this work.
Funding sources for the study: National Science Fund of China (81300815 to J.M., and 81570310000 to S.L.), the 12th Five‐Year Science and Technology Support Program (2014BAI07B04 to J.M.); international cooperation project of Sichuan Provincial Science and Technology Department (2018HH0097 to J.M.); Program of Sichuan Provincial Department of Education (18ZB0213 to W.G.); and Youth Innovation Project of Sichuan Provincial Medical Association (Q15025 to W.G.).
Potential conflict of interest: None provided.
The data presented herein were analyzed in part using free online cloud platform Majorbio I‐Sanger (www.i-sanger.com).
Read the full text
ePDFPDFTOOLS SHARE
Abstract
Background
Little is known regarding the role of the microbiome of the paranasal sinuses and its contribution to sinus mucosal health and disease. Consequently, we examined the microbiome of chronic rhinosinusitis patients with polyps (CRSwNP) and a control population to provide new insights into the microbiota associated with the pathogenesis of CRSwNP.
Methods
Fifty‐nine CRSwNP patients and 27 controls were enrolled in the study. The bacterial communities of the middle meatus were detected using 16S ribosomal RNA (rRNA)‒targeted Illumina MiSeq sequencing after microbial DNA was extracted from swabs.
Results
Although there was no difference in diversity between the 2 groups, richness was lower in the CRSwNP group than in the control group (p = 0.03). At the phylum level, Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes were predominant in both groups; however, the relative abundance was different, with the proportions of Actinobacteria (predominantly Corynebacterium) and Dolosigranulum being significantly higher in the control group than in the CRSwNP group.
Conclusion
These results support the theory of microbial dysbiosis as the pathogenesis of CRSwNP. The reduction in the proportions of potentially protective bacteria may decrease the overall stability of the sinonasal bacterial community.
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