Patterns of immune-cell infiltration in murine models of melanoma: roles of antigen and tissue site in creating inflamed tumorsAbstract
Immune-cell infiltration is associated with improved survival in melanoma. Human melanoma metastases may be grouped into immunotypes representing patterns of immune-cell infiltration: A (sparse), B (perivascular cuffing), and C (diffuse). Immunotypes have not been defined for murine melanomas, but may provide opportunities to understand mechanism-driving immunotype differences. We performed immunohistochemistry with immune-cell enumeration, immunotyping, and vascular density scoring in genetically engineered (Braf/Pten and Braf/Pten/β-catenin) and transplantable (B16-F1, B16-OVA, and B16-AAD) murine melanomas. The transplantable tumors were grown in subcutaneous (s.c.) or intraperitoneal (i.p.) locations. Braf/Pten and Braf/Pten/β-catenin tumors had low immune-cell densities, defining them as Immunotype A, as did B16-F1 tumors. B16-OVA (s.c. and i.p.) and B16-AAD s.c. tumors were Immunotype B, while B16-AAD i.p. tumors were primarily Immunotype C. Interestingly, the i.p. location was characterized by higher immune-cell counts in B16-OVA tumors, with counts that trended higher for B16-F1 and B16-AAD. The i.p. location was also characterized by higher vascularity in B16-F1 and B16-AAD tumors. These findings demonstrate that spontaneously mutated neoantigens in B16 melanomas were insufficient to induce robust intratumoral immune-cell infiltrates, but instead were Immunotype A tumors. The addition of model neoantigens (OVA or AAD) to B16 enhanced infiltration, but this most often resulted in Immunotype B. We find that tumor location may be an important element in enabling Immunotype C tumors. In aggregate, these data suggest important roles both for the antigen type and for the tumor location in defining immunotypes.
|
ΩτοΡινοΛαρυγγολόγος Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,
Translate
Ετικέτες
Δευτέρα 24 Ιουνίου 2019
Αναρτήθηκε από
Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
στις
10:47 μ.μ.
Ετικέτες
00302841026182,
00306932607174,
alsfakia@gmail.com,
Anapafseos 5 Agios Nikolaos 72100 Crete Greece,
Medicine by Alexandros G. Sfakianakis
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Αρχειοθήκη ιστολογίου
-
►
2023
(278)
- ► Φεβρουαρίου (139)
- ► Ιανουαρίου (139)
-
►
2022
(1962)
- ► Δεκεμβρίου (107)
- ► Σεπτεμβρίου (158)
- ► Φεβρουαρίου (165)
- ► Ιανουαρίου (163)
-
►
2021
(3614)
- ► Δεκεμβρίου (152)
- ► Σεπτεμβρίου (271)
- ► Φεβρουαρίου (64)
- ► Ιανουαρίου (357)
-
►
2020
(3279)
- ► Δεκεμβρίου (396)
- ► Σεπτεμβρίου (157)
- ► Φεβρουαρίου (382)
- ► Ιανουαρίου (84)
-
▼
2019
(11718)
- ► Δεκεμβρίου (265)
- ► Σεπτεμβρίου (545)
-
▼
Ιουνίου
(2501)
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- Environmental Science and Pollution Research
- Der Hautarzt
- Medizinrecht
- Orofacial Orthopedics / Fortschritte der Kieferort...
- Mycopathologia
- Molecular & Cellular Toxicology
- Sexual Behavior
- Food Science and Biotechnology
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- Synthese
- Immunopathology
- Diabetology
- Molecular Histology
- Sophia
- DGNeurologie
- Designs, Codes and Cryptography
- Stochastic Environmental Research and Risk Assessment
- Biogerontology
- Gastroenterology
- Cancer Education
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- Psycho-Pharmacology
- Cardiovascular Drugs
- Natural Medicines
- Pediatric Drugs
- Neurosurgical Anesthesiology
- Transplantation
- Psycho-Pharmacology
- Gastrointestinal Surgery
- Otolaryngology
- Strahlentherapie und Onkologie
- History and Philosophy of the Life Sciences
- Law and Philosophy
- Hematology
- Animal Microbiome
- CardioVascular and Interventional Radiology
- Climatic Change
- Chromatographia
- Integrative Medicine
- AUMENTO DA PREVALÊNCIA DE ANGIODISPLASIAS NA ENTE...
- PAQUIMENINGITIS HIPERTRÓFICA POSINFECCIOSA: PRESE...
- EL AMPARO ECONOMICO PARA MEJORAR EL TRATAMIENTO D...
- Philosophical Studies
- Neuro-Oncology
- Medicina Interna
- Los péptidos antimicrobianos son moléculas con una...
- Cirugía Ortopédica y Traumatología
- Acupuncture and Tuina Science
- Medicinal Chemistry Research
- General Thoracic and Cardiovascular Surgery
- Brain Structure and Function
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- Paediatric Dermatology
- Allergy and Clinical Immunology
- Buddhists Care: Examining the Impact of Religiou...
- Invasive and Non-Invasive Fungal Rhinosinusitis...
- IJMS, Vol. 20, Pages 3182: Renin Activity in...
- International Journal of Environmental Research an...
- Humanities, Vol. 8, Pages 118: A Mind Trying...
- Heritage, Vol. 2, Pages 1748-1761: Contempor...
- Healthcare, Vol. 7, Pages 82: The Power of M...
- Geriatrics, Vol. 4, Pages 40: Geriatric Asse...
- GastrointestDisord, Vol. 1, Pages 290-300: I...
- Fractal Fract, Vol. 3, Pages 37: Inequalitie...
- Foods, Vol. 8, Pages 231: Use of Attenuated ...
- Fermentation, Vol. 5, Pages 52: Solid State ...
- European Journal of Investigation in Health, Psych...
- Epigenomes, Vol. 3, Pages 12: Polycomb Assem...
- Environments, Vol. 6, Pages 75: Current Stat...
- Diversity, Vol. 11, Pages 102: New Material ...
- Diseases, Vol. 7, Pages 47: Cell and Gene Therap...
- Diagnostics, Vol. 9, Pages 66: In vivo Diagnosis...
- Dentistry Journal, Vol. 7, Pages 64: Impact of D...
- Cryptography, Vol. 3, Pages 16: I2PA: An Efficie...
- Cosmetics, Vol. 6, Pages 36: Unique Hair Propert...
- Clocks & Sleep, Vol. 1, Pages 280-289: How to Re...
- Climate, Vol. 7, Pages 84: The Effect of Agulhas...
- Clean Technol., Vol. 1, Pages 141-153: Optimizat...
- Children, Vol. 6, Pages 81: Effects of Gestation...
- Cells, Vol. 8, Pages 649: Nuclear Phosphoinositi...
- Cancers, Vol. 11, Pages 911: Decoding Immune Het...
- Carbon research
- Brain Sciences, Vol. 9, Pages 154: Neural Sensit...
- Biomolecules, Vol. 9, Pages 255: Site-Specific I...
- Biomimetics, Vol. 4, Pages 43: Matrix Nanopatter...
- Biomedicines, Vol. 7, Pages 46: Protein Nanotube...
- Biology, Vol. 8, Pages 52: The Use of Myelinatin...
- Bioengineering, Vol. 6, Pages 55: Influence of P...
- Beverages, Vol. 5, Pages 40: Biogenic Amines Det...
- Behavioral Sciences, Vol. 9, Pages 69: The Push ...
- Atmosphere, Vol. 10, Pages 358: Dust-Associated ...
- Arts, Vol. 8, Pages 77: The Painting Industries ...
- Antioxidants, Vol. 8, Pages 200: Optimization of...
- Antibodies, Vol. 8, Pages 38: Super Potent Bispe...
- Antibiotics, Vol. 8, Pages 85: Antimicrobial Res...
- Acoustics, Vol. 1, Pages 450-472: Wave Mode Iden...
- ► Φεβρουαρίου (1143)
- ► Ιανουαρίου (744)
-
►
2017
(2)
- ► Φεβρουαρίου (1)
- ► Ιανουαρίου (1)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου