Molecular detection of fungal and bacterial DNA from pustules in patients with palmoplantar pustulosis: special focus on Malassezia species
Y. Matsumoto K. Harada T. Maeda C. Egusa H. Hirano Y. Okubo R. Tsuboi
First published: 20 June 2019 https://doi.org/10.1111/ced.14026
This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1111/ced.14026
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Abstract
Background
Palmoplantar pustulosis (PPP) is a distinct, chronic skin disorder characterized by intraepidermal pustules on the palms and soles. It is hypothesized that microorganisms on the skin might induce the symptoms of PPP via inflammatory cell activation. However, the microbiota has not been studied in detail because of the assumption that the pustules in PPP are sterile.
Aim
To elucidate the role of microorganisms in pathogenesis of PPP.
Methods
We employed a PCR analysis of microbial DNA fragments in the pustules of patients with PPP. The sequence of the D1/D2 LSU 26s rRNA gene and that of the 16S rRNA gene was used for fungal and bacterial DNA detection, respectively.
Results
In total, 71 samples were carefully collected from the pustules of the PPP patients. Fungal DNA bands were detected in 68 samples, and fungi including Malassezia spp. were identified in 30 of 71 samples (42.3%). M. restricta was the most frequently encountered fungus (14/71, 19.7%). However, bacterial DNA was not detected by the methods used. Furthermore, identical fungal DNA was not detected in the outer lid of the pustules, suggesting that the fungi detected within the pustule did not derive from contamination via the skin surface.
Conclusions
In the present study, we demonstrated for the first time that certain pustules in PPP patients contain fungal DNA fragments, especially those of Malassezia spp. Our findings provide new insights on the role of skin microbiota in the pathogenesis of PPP.
Y. Matsumoto K. Harada T. Maeda C. Egusa H. Hirano Y. Okubo R. Tsuboi
First published: 20 June 2019 https://doi.org/10.1111/ced.14026
This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1111/ced.14026
ePDFPDFTOOLS SHARE
Abstract
Background
Palmoplantar pustulosis (PPP) is a distinct, chronic skin disorder characterized by intraepidermal pustules on the palms and soles. It is hypothesized that microorganisms on the skin might induce the symptoms of PPP via inflammatory cell activation. However, the microbiota has not been studied in detail because of the assumption that the pustules in PPP are sterile.
Aim
To elucidate the role of microorganisms in pathogenesis of PPP.
Methods
We employed a PCR analysis of microbial DNA fragments in the pustules of patients with PPP. The sequence of the D1/D2 LSU 26s rRNA gene and that of the 16S rRNA gene was used for fungal and bacterial DNA detection, respectively.
Results
In total, 71 samples were carefully collected from the pustules of the PPP patients. Fungal DNA bands were detected in 68 samples, and fungi including Malassezia spp. were identified in 30 of 71 samples (42.3%). M. restricta was the most frequently encountered fungus (14/71, 19.7%). However, bacterial DNA was not detected by the methods used. Furthermore, identical fungal DNA was not detected in the outer lid of the pustules, suggesting that the fungi detected within the pustule did not derive from contamination via the skin surface.
Conclusions
In the present study, we demonstrated for the first time that certain pustules in PPP patients contain fungal DNA fragments, especially those of Malassezia spp. Our findings provide new insights on the role of skin microbiota in the pathogenesis of PPP.
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