Investigation the effect of Hypericum Perforatum on corneal alkali burns
Uğur Yılmaz, Hüseyin Kaya, Murat Turan, Ferda Bir & Barbaros Şahin
Received 07 Jan 2019, Accepted 20 May 2019, Accepted author version posted online: 28 May 2019
Download citation  https://doi.org/10.1080/15569527.2019.1622560 
 
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Abstract
Purpose: To investigate the effect of Hypericum Perforatum on corneal alkali burn.

Method: We studied 45, 250 grams weighing, 4 months old Wistar Albino rats. Alkaline burns were performed in the corneas of all experimental animals with 2 mol/L NaOH after general anesthesia. Rats were divided into five groups according to the subsequent process applied to them: group 1 was the topical Hypericum perforatum group, group 2 was the topical pure olive oil group, group 3 was the oral Hypericum perforatum group, group 4 was the oral pure olive oil group and group 5 was the control untreated group. Rats were sacrificed under general anesthesia on the fourteenth day. The rate of corneal inflammation, neovascularization, fibroblastic activity, and CD31 staining was investigated.

Result: There were 45 rats at the beginning of the study. One rat in group 1, 2, and 3 died during the study; therefore, 42 rats could be evaluated. There were 8 rats in group 1, 8 rats in group 2, 9 rats in group 3, and 8 rats in group 4. We found less CNV, inflammation, and fibroblastic activity in group 1 and group 2 than in the other groups(p˂0.001 for all parameters).

CNV, inflammation, fibroblastic activity, and CD31 staining rates were lower in group 1 than in group 2(p˂0.001 for all parameters). There was no difference between groups 3, 4, and 5(respectively, p = 0.436, 0.634, 0.750).

Conclusion: We found that both topical Hypericum perforatum oily extract and olive oil have anti-inflammatory, anti-angiogenic and anti-fibroblastic effects when applied after corneal alkali burns in rat corneas. Further studies should be conducted in this field.

Keywords: Corneal alkali burn, Hypericum Perforatum, Olive oil, corneal vascularization, corneal inflammation, corneal fibroblastic activity