[ASAP] Hierarchically Porous S/N Codoped Carbon Nanozymes with Enhanced Peroxidase-like Activity for Total Antioxidant Capacity Biosensing

[ASAP] Hierarchically Porous S/N Codoped Carbon Nanozymes with Enhanced Peroxidase-like Activity for Total Antioxidant Capacity Biosensing:

Abstract

Design of highly active carbon nanozymes and further establishment of ultrasensitive biosensors remain a challenge. Herein, hierarchically porous carbon nanozymes with sulfur (S)/nitrogen (N) codoping (SNC) were developed. Compared with N-doped carbon (NC) nanozymes, SNC nanozymes have a smaller Michaelis–Menten constant and higher specific activities, demonstrating that the S-doping in SNC nanozymes could not only enhance their affinity toward substrates but also improve their catalytic performance. These results may be caused by the synergistic effect of heteroatoms (S and N). Because of the good enzyme-like activity, the proposed SNC nanozymes were exploited to the colorimetric detection of the total antioxidant capacity (TAC) using ascorbic acid as a typical model with a limit of detection of 0.08 mM. Because of its high sensitivity and selectivity and encouraging performance, the detection method presented practical feasibility for the TAC assay in commercial beverages. This work paves a way to design the highly active carbon nanozymes and expand their applications in the construction of high-performance biosensors.

Supporting Information

---

The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.0c02982.

• TEM of NC nanozymes; XRD and XPS of NC and SNC nanozymes; BET and Raman spectra of SNC-WZ and SNC-WSZ nanozymes; influences of pH and temperature toward the POD-like activities of NC, SNC nanozymes, and HRP; and dynamics data of NC and SNC nanozymes (PDF)

• pdf

  • ac0c02982_si_001.pdf (854.07 kb)

Analytical Chemistry

DOI: 10.1021/acs.analchem.0c02982