Increased Retention of Gadolinium in the Inflamed Brain After Repeated Administration of Gadopentetate Dimeglumine: A Proof-of-Concept Study in Mice Combining ICP-MS and Micro– and Nano–SR-XRF Objectives The aim of this study was to determine in vivo if brain inflammation leads to increased gadolinium (Gd) retention in brain tissue after repeated applications of Gd-based contrast agents (GBCAs). Materials and Methods Experimental autoimmune encephalomyelitis (EAE) was induced in female SJL/J mice (n = 6). Experimental autoimmune encephalomyelitis and healthy control mice (n = 4) received 2.5 mmol/kg Gd-DTPA over 10 days (8 injections, cumulated dose of 20 mmol/kg), starting at day 14 post immunization when EAE mice reached the maximal clinical disability. In a group of mice, T1-weighted 2-dimensional RARE images were acquired before the first GBCA injection and 1 day after the last injection. Mice were killed either 1 day or 10 days after the last Gd application. From each single animal, a brain hemisphere was used for Gd detection using inductively coupled plasma mass spectrometry, whereas the other hemisphere was processed for histology and synchrotron x-ray fluorescence spectroscopy (SR-XRF) analysis. Results Gadolinium deposition in inflamed brains was mapped by SR-XRF 1 day after the last Gd-DTPA injections, although only mild signal hyperintensity was found on unenhanced T1-weighted images. In addition, using inductively coupled plasma mass spectrometry, we detected and quantified Gd in both healthy and EAE brains up to 10 days after the last injections. However, EAE mouse brains showed higher levels of Gd (mean ± SD, 5.3 ± 1.8 μg/g; range, 4.45–8.03 μg/g) with respect to healthy controls (mean ± SD, 2.4 ± 0.6 μg/g; range, 1.8–3.2 μg/g). By means of micro–SR-XRF, we identified submicrometric Gd hotspots in all investigated samples containing up to 5893 μg Gd/g tissue. Nano–SR-XRF further indicated that Gd small hotspots had an average size of ~160 nm diameter and were located in areas of high inflammatory activity. Conclusions After repeated administrations of Gd-DTPA, ongoing inflammation may facilitate the retention of Gd in the brain tissue. Thus, neuroinflammation should be considered as a risk factor in the recommendation on use of linear GBCA-enhanced MRI. Received for publication December 14, 2018; and accepted for publication, after revision, March 11, 2019. Shuangqing Wang and Bernhard Hesse contributed equally to this article. Conflicts of interest and sources of funding: There are no conflicts of interest to declare. This work was supported by the European Synchrotron Radiation Facility and the German Research Foundation (Deutsche Forschungsgemeinschaft; SFB1340-1/B05, IN156/4-1, and GRK2260 [BIOQIC]). Supplemental digital contents are available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal’s Web site (www.investigativeradiology.com). Correspondence to: Carmen Infante-Duarte, PhD, Experimental Neuroimmunology, Institute for Medical Immunology, Charité–Universitätsmedizin Berlin, Campus Virchow Klinikum, Augustenburger Platz 1, 13353 Berlin, Germany. E-mail: carmen.infante@charite.de. Copyright © 2019 Wolters Kluwer Health, Inc. All rights reserved. |
ΩτοΡινοΛαρυγγολόγος Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,
Translate
Ετικέτες
Πέμπτη 23 Μαΐου 2019
Αναρτήθηκε από
Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
στις
5:54 π.μ.
Ετικέτες
00302841026182,
00306932607174,
alsfakia@gmail.com,
Anapafseos 5 Agios Nikolaos 72100 Crete Greece,
Medicine by Alexandros G. Sfakianakis
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Αρχειοθήκη ιστολογίου
-
►
2023
(278)
- ► Φεβρουαρίου (139)
- ► Ιανουαρίου (139)
-
►
2022
(1962)
- ► Δεκεμβρίου (107)
- ► Σεπτεμβρίου (158)
- ► Φεβρουαρίου (165)
- ► Ιανουαρίου (163)
-
►
2021
(3614)
- ► Δεκεμβρίου (152)
- ► Σεπτεμβρίου (271)
- ► Φεβρουαρίου (64)
- ► Ιανουαρίου (357)
-
►
2020
(3279)
- ► Δεκεμβρίου (396)
- ► Σεπτεμβρίου (157)
- ► Φεβρουαρίου (382)
- ► Ιανουαρίου (84)
-
▼
2019
(11718)
- ► Δεκεμβρίου (265)
- ► Σεπτεμβρίου (545)
-
▼
Μαΐου
(1970)
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- Pulmonary pleomorphic carcinoma harboring epide...
- Cervical cancer with breast metastasisChing-Tin...
- Inflammatory Pseudotumor of the LiverTsen-Long ...
- Pembrolizumab-induced uveitis in a patient with...
- Detection of anaplastic lymphoma kinase gene re...
- A Patient with refractory metastatic germ cell ...
- Modulation of oxidative stress by doxorubicin l...
- Antiproliferative effect of oxidative stress in...
- High-intensity focused ultrasound for localized...
- Prevention and management catheter-associated uri...
- people suffering from special phobiasAlireza Sang...
- singing on the happiness of older peopleMaryam En...
- Modeling of cardiac function of patients with a...
- patients with spinal muscular atrophy ,Ali-Asghar...
- Effect of cognitive behavior therapy integrated w...
- Warning System Designed to Predict Severe Sepsis ...
- Neurologic Complications of Infective Endocardi...
- Myeloid-Derived Suppressor Cells Mediate Immunosu...
- Tumour Spheroids-on-a-Chip ,M Ibrahim Khot, Mark ...
- Cancer Research and Practice
- Nursing and Midwifery Sciences
- Critical Care Medicine
- Podcast: Being a Professional Musician with Bipola...
- Medicine by Alexandros G. Sfakianakis,Anapafseos 5...
- Adjuvant therapies in venous leg ulcer management...
- Pressure Ulcer Summit 2018: An Interdisciplinary ...
- Leukemia inhibitory factor promotes the regenerat...
- Toll like receptors (TLRs) in response to human...
- Evaluation of hypercalciuria in patients receiv...
- Cytochemical tests to investigate sperm DNA dam...
- Family Medicine and Primary Care
- IL‐17A is not a treatment target in progressiv...
- The next‐generation BET inhibitor, PLX51107, d...
- Otolaryngology & Head and Neck Surgery
- Tropical Medicine
- Treating the primary in metastatic prostate cance...
- Cytoreductive nephrectomy in metastatic kidney ...
- Treating advanced penile cancer: where do we st...
- Supplemental oxygen for the management of dyspn...
- Multidimensional measurement of breathlessness:...
- Interdisciplinary management of chronic breath...
- Supportive and Palliative Care
- Organ Transplantation
- Serum carcinoembryonic antigen to predict recurre...
- Precision medicine in the ageing world: The role ...
- Prognostic roles of microRNA 143 and microRNA 145...
- Elevated neutrophil to lymphocyte and platelet to...
- Associated measurement of fucosylated levels of A...
- Clinicopathological and prognostic significance o...
- β-Arrestin-1 expression and epithelial-to-mesench...
- Prognostic nomogram for patients with non-metasta...
- Clinicopathological and molecular differences in ...
- Performance of a MethyLight assay for methylated ...
- Cell-free microRNAs as non-invasive biomarkers in...
- Urinary markers in treatment monitoring of lung c...
- Circular RNA ABCB10 promotes tumor progression an...
- Predictive significance of CYFRA21-1, squamous ce...
- Clinical significance of tumor miR-21, miR-221, m...
- The serum level of CC chemokine ligand 18 correla...
- iTRAQ-based proteomics reveals SOD2 as a potentia...
- New and old biomarkers in the differential diagno...
- Prognostic markers affecting the early recurrence...
- Cancer registries - guardians of breast cancer bi...
- Automated Pupillometry Devices in Critically Il...
- Language Monitoring in Brain Surgery Under Gene...
- Subanesthetic Dose of Ketamine Improved CFA-ind...
- Defining a Taxonomy of Intracranial Hypertensio...
- Pain Anxiety as a Mechanism Linking Pain Severi...
- The Association Between Antenatal Maternal Self...
- Prenatal Practice Staff Perceptions of Three Su...
- Exploring the Effects of Vitamin D Supplementat...
- How Might Maternal Poverty Impact the Course of...
- Poverty and Excess Length of Hospital Stay in Neo...
- The Effect of Depth of Anesthesia on Hemodynam...
- Neurosurgical Anesthesiology
- Addiction Medicine
- Vehicle Collision : Blunt rupture of the myocardiu...
- Variability in international normalized ratio a...
- Promotility Agents For The Treatment Of Ileus I...
- Ketamine Infusion for Pain Control in Adult Pat...
- Resuscitative Endovascular Balloon Occlusion of...
- Trauma and Acute Care Surgery
- Unfractionated heparin attenuates histone-mediat...
- Wall Contrast Enhancement of Thrombosed Intracran...
- Assessment of Explicitly Stated Interval Change o...
- Deep Learning–Based Automatic Segmentation of Lum...
- Hazardous Materials
- Sugar Alternatives : Molasses,Raw Honey,Stevia,Xyl...
- The Hearing Journal
- Molecular markers used to guide treatment, assess ...
- Programmed death-ligand 1 (PD-L1) also known as cl...
- Anaplastic lymphoma kinase (ALK) also known as ALK...
- Proto-oncogene tyrosine-protein kinase ROS is an e...
- The KRAS gene provides instructions for making a p...
- Amplification, also known as the over-expression o...
- Mutations that lead to EGFR overexpression (known ...
- A BRCA mutation is a mutation in either of the BRC...
- BRAF is a human gene that encodes a protein called...
- Differences in the Intended Meaning of Congest...
- ► Φεβρουαρίου (1143)
- ► Ιανουαρίου (744)
-
►
2017
(2)
- ► Φεβρουαρίου (1)
- ► Ιανουαρίου (1)
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου