[Evaluation of novel nucleic acid detection kit for Mycoplasma pneumoniae].

[Article in Japanese]

Shinto K1, Kato K1, Narita T1, Hanaiwa H1, Harada T1, Miyako K2, Funashima Y3, Sato K3, Nagasawa Z3, Umemura T4.

Author information

1

Department of Clinical Laboratory, Medical Kouhoukai Takagi Hospital.

2

Department of Clinical Laboratory, Medical Kouhoukai Takagi Hospital. Medical Genome Institute, International University of Health and Welfare.

3

Department of Medical Technology and Science, Faculty of Fukuoka Health Care, International University of Health and Welfare.

4

Department of Clinical Laboratory, Medical Kouhoukai Takagi Hospital. Department of Medical Technology and Science, Faculty of Fukuoka Health Care, International University of Health and Welfare.

Abstract

For diagnosis of Mycoplasma pneumoniae infection, highly sensitive and rapid diagnosis is important. Because antibiotics are limited for the treatment of M. pneumoniae infection. In this study, we evaluated new rapid nucleic acid detection kit for M. pneumoniae. This kit does not require excessive pretreatment of specimens and molecular diagnosis of M. pneumoniae is possible within 40 min. Using 120 nasopharyngeal specimens, we compared this kit with a commercially available molecular diagnostic reagent (LAMP). 51 of 120 cases were M. pneumoniae positive, and the results of both assays were all consistent. In addition, sequencing of 23S rRNA gene was performed on 51 cases positive for M. pneumoniae. As a result, macrolide resistance mutation (2063A>G) was observed in 19 cases (37.3%). The gene mutations estimated by this kit coincided completely with the sequencing. In conclusion, new rapid nucleic acid detection kit could detect M. pneumoniae with the same sensitivity as other molecular diagnostics, in a simple process.

PMID:

 

30630334

[Indexed for MEDLINE]