In silico analysis of RNA-seq requires a more complete description of methodology     Joël Simoneau & Michelle S. Scott pp451 - 452 | doi:10.1038/s41580-019-0137-z Simoneau and Scott ask that researchers provide complete descriptions of their choices and parameters of RNA-seq data analysis.  Full Text | PDF
RESEARCH HIGHLIGHTS
ER stress boosts respiration  Paulina Strzyz p453 | doi:10.1038/s41580-019-0139-x Glucose deprivation induces ER stress, which enhances mitochondrial respiration by promoting the assembly of respiratory supercomplexes.  PDF  Embryonic hydraulics  Paulina Strzyz p454 | doi:10.1038/s41580-019-0154-y Hydraulic forces have important roles in the early development of mouse embryos.  PDF  Histone methylation boosts liver regeneration  Eytan Zlotorynski pp454 - 455 | doi:10.1038/s41580-019-0157-8 Upon loss of liver DNA methylation, H3K27me3 is redistributed from pro-regeneration genes to hypomethylated transposons, thereby potentiating liver regeneration.  PDF  Tracing cell fate  Katharine H. Wrighton pp454 - 455 | doi:10.1038/s41580-019-0148-9 A new CRISPR–Cas9-based ‘molecular recorder’ can provide information on cell state and cell lineage, in mice, from fertilization through to adulthood.  PDF  The rise of a forgotten model  Henrik Jönsson p455 | doi:10.1038/s41580-019-0144-0 Henrik Jönsson discusses the mathematical model for hormone movement through plant tissues that Graeme Mitchison proposed in 1980, and how models can inspire new research.  PDF
REVIEWS
Integrins as biomechanical sensors of the microenvironment     Jenny Z. Kechagia, Johanna Ivaska & Pere Roca-Cusachs pp457 - 473 | doi:10.1038/s41580-019-0134-2 Integrin extracellular matrix receptors establish contacts between the cell interior and the cell microenvironment. Integrins are subjected to complex biochemical and mechanical regulation, which allows cells to respond to extracellular matrix with different physicochemical properties and fine-tunes cell behaviour.  Full Text | PDF
The roles of structural dynamics in the cellular functions of RNAs     Laura R. Ganser, Megan L. Kelly, Daniel Herschlag & Hashim M. Al-Hashimi pp474 - 489 | doi:10.1038/s41580-019-0136-0 The functions of many regulatory RNAs depend on how their 3D structure changes in response to cellular conditions. Recent studies have revealed that RNA exists as a dynamic ensemble of conformations, which form with different probabilities in different cellular conditions and thus modulate RNA function.  Full Text | PDF
The next generation of CRISPR–Cas technologies and applications     Adrian Pickar-Oliver & Charles A. Gersbach pp490 - 507 | doi:10.1038/s41580-019-0131-5 CRISPR–Cas systems have revolutionized genome editing, and the CRISPR–Cas toolkit has been expanding to include single-base editing enzymes, targeting RNA and fusing inactive Cas proteins to effectors that regulate various nuclear processes. Consequently, CRISPR–Cas systems are being tested for gene and cell therapies.  Full Text | PDF
AMENDMENTS & CORRECTIONS
Author Correction: Functions and mechanisms of non-histone protein acetylation     Takeo Narita, Brian T. Weinert & Chunaram Choudhary p508 | doi:10.1038/s41580-019-0156-9 Full Text | PDF