Effect of Taxifolin on Methanol Induced Oxidative and Inflammatory Optic Nerve Damage in Rats
Ibrahim Ahiskali, Can Lokman Pinar, Murat Kiki, Murat Cankaya, Celaleddin Semih Kunak & Durdu Altuner
Received 28 May 2019, Accepted 24 Jun 2019, Accepted author version posted online: 27 Jun 2019
Download citation https://doi.org/10.1080/15569527.2019.1637348
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Abstract
Purpose: Oxidative stress and inflammation have been demonstrated in the pathogenesis of methanol toxicity. Taxifolin has antioxidant and anti-inflammatory properties. In this study, we examined the protective effect of taxifolin against methanol-induced optic nerve toxicity. Materials and Methods: Animals were divided into four groups (n = Six): healthy control group (HG), methotrexate treated group (MTX), methotrexate + methanol treated group (MTX + M), and methotrexate + methanol + taxifolin treated group (MTX + M+T). Methotrexate was administered to all groups except HG group 3 mg/kg via oral gavage for 7 days. After that 20% methanol was orally administered to the MTX + M and MTX + M+T group at a dose of 3 g/kg. After four hours, taxifolin was orally administered to MTX + M+T group 50 mg/kg. Animals were sacrificed by high-dose thiopental anesthesia 8 hours after taxifolin administration, and biochemical studies were performed. Results: Malondialdehyde, total oxidant system, nuclear factor kappa-B and tumor necrosis factor-alpha levels were significantly higher in the optic nerve of MTX and MTX + M groups compared to HG group. Otherwise total glutathione and total antioxidant system levels decreased in MTX and MTX + M groups according to the HG group. Malondialdehyde, total oxidant system, nuclear factor kappa-B and tumor necrosis factor-alpha levels were decreased in the MTX + M+T group and total glutathione and total antioxidant system levels increased in the MTX + M+T group according to the MTX + M group. Conclusions: These results indicate that taxifolin prevents oxidative and inflammatory optic nerve damage due to methanol exposure.
Keywords: taxifolin, methanol, optic nerve, damage
Ibrahim Ahiskali, Can Lokman Pinar, Murat Kiki, Murat Cankaya, Celaleddin Semih Kunak & Durdu Altuner
Received 28 May 2019, Accepted 24 Jun 2019, Accepted author version posted online: 27 Jun 2019
Download citation https://doi.org/10.1080/15569527.2019.1637348
Select Language▼
Translator disclaimer
Accepted author version
Abstract
Purpose: Oxidative stress and inflammation have been demonstrated in the pathogenesis of methanol toxicity. Taxifolin has antioxidant and anti-inflammatory properties. In this study, we examined the protective effect of taxifolin against methanol-induced optic nerve toxicity. Materials and Methods: Animals were divided into four groups (n = Six): healthy control group (HG), methotrexate treated group (MTX), methotrexate + methanol treated group (MTX + M), and methotrexate + methanol + taxifolin treated group (MTX + M+T). Methotrexate was administered to all groups except HG group 3 mg/kg via oral gavage for 7 days. After that 20% methanol was orally administered to the MTX + M and MTX + M+T group at a dose of 3 g/kg. After four hours, taxifolin was orally administered to MTX + M+T group 50 mg/kg. Animals were sacrificed by high-dose thiopental anesthesia 8 hours after taxifolin administration, and biochemical studies were performed. Results: Malondialdehyde, total oxidant system, nuclear factor kappa-B and tumor necrosis factor-alpha levels were significantly higher in the optic nerve of MTX and MTX + M groups compared to HG group. Otherwise total glutathione and total antioxidant system levels decreased in MTX and MTX + M groups according to the HG group. Malondialdehyde, total oxidant system, nuclear factor kappa-B and tumor necrosis factor-alpha levels were decreased in the MTX + M+T group and total glutathione and total antioxidant system levels increased in the MTX + M+T group according to the MTX + M group. Conclusions: These results indicate that taxifolin prevents oxidative and inflammatory optic nerve damage due to methanol exposure.
Keywords: taxifolin, methanol, optic nerve, damage
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