Abstract Introduction Expectant and parenting teens experience many challenges to achieving self-sufficiency and promoting their children’s healthy development. Teen parents need support to help them address these challenges, and many different types of programs aim to support them. In this systematic review, we examine the research about programs that aim to support aspects of teen parents’ self-sufficiency by promoting their educational...
1h
ObjectiveHandoffs are often framed as the co-construction of a shared understanding relying on narrative storytelling. We investigated how narratives are constructed and used during resident and nurse handoff conversations. MethodWe audio-recorded resident (n=149) and nurse (n=126) handoffs in an inpatient medicine unit. Qualitative analysis using grounded theory was conducted to identify and characterise the structure of resident and nursing handoff narratives. ResultsHandoff conversations among...
1h
Current methods used to evaluate the effects of healthcare improvement efforts have limitations. Designs with strong causal inference—such as individual patient or cluster randomisation—can be inappropriate and infeasible to use in single-centre settings. Simpler designs—such as prepost studies—are unable to infer causal relationships between improvement interventions and outcomes of interest, often leading to spurious conclusions regarding programme success. Other designs, such as regression discontinuity...
1h
IntroductionWe all grow old. Even surgeons. We slow down, we weaken and our skills diminish. Although individuals differ and chronological age may not be an accurate guide to biological age, we cannot hold back the advancing years. How long should we allow surgeons to keep operating? If public safety is the priority, as it must be, should there be a mandatory retirement age, as there is for pilots in the airline industry? Or is there a fair and equitable way of assessing those nearing the end of...
1h
There is wide recognition that promoting healthcare value involves decreasing ‘low-value’ services—care without clinical benefit, little benefit compared with cost or disproportionate potential harm.1 While low-value care has been presumed to be a problem predominantly in the USA in the context of an expensive, fragmented, multipayer, fee-for-service system, recent evidence suggests low-value services are pervasive even in government-funded healthcare systems with universal coverage and interoperability.2...
1h
Effective teamwork and communication is now recognised as a critical component of safe and high-quality patient care. Researchers, policymakers and frontline providers are in search of strategies to improve teamwork in healthcare. The most frequently used strategy is teamwork training.1 Teamwork training involves a systematic process in which a team is guided (often by facilitators) to improve and master different skills for working together effectively. Single-centre teamwork training initiatives...
1h
In USA, cancer outcomes have steadily improved but considerable disparities in outcomes persist.1 There is continued evidence that vulnerable patients (ie, those who are socially or economically disadvantaged) are less likely to receive high-quality care and subsequently have poorer outcomes.2 Since the release of the Institute of Medicine’s report Ensuring Quality Cancer Care in 1999, increased attention has been paid to the importance of measuring cancer care quality, understanding its effects...
1h
BackgroundChemotherapy quality measures consider hospitals compliant when chemotherapy is recommended, even if it is not received. This may mask shortcomings in cancer care delivery. Objectives of this study were to (1) identify patient factors associated with failure to receive recommended chemotherapy without a documented contraindication and (2) assess hospital variation in failure to administer recommended chemotherapy. MethodsPatients from 2005 to 2015 with breast, colon and lung cancers who...
1h
ObjectivesTo develop and validate a tool to predict the risk of an older adult experiencing medication-related harm (MRH) requiring healthcare use following hospital discharge. Design, setting, participantsMulticentre, prospective cohort study recruiting older adults (≥65 years) discharged from five UK teaching hospitals between 2013 and 2015. Primary outcome measureParticipants were followed up for 8 weeks in the community by senior pharmacists to identify MRH (adverse drug reactions, harm from...
1h
BackgroundUnlike some other safety critical professions, there is no mandatory age of retirement for doctors, including surgeons. Medical regulators in Australia are implementing additional checks on doctors from the age of 70. We describe expert opinions on assuring performance and supporting career transitions among older surgeons. MethodsIn this qualitative study, experts in four countries were purposively selected for their expertise in surgical governance. Experts responded to interviews (Australia,...
1h
Clostridioides difficile infection (CDI) remains an important healthcare-associated infection and threat to patient safety since the height of the NAP1/027 epidemic in the early part of the millennium. In 2011, C. difficile caused almost half a million infections and 29 000 deaths in the USA alone, with 24% of those cases occurring in hospital settings.1 The US Centres for Disease Control identifies C. difficile as one of three pathogens that poses ‘an immediate antibiotic resistance threat that...
1h
ObjectiveTo assess whether the implementation of an intrapartum training package (PROMPT (PRactical Obstetric Multi-Professional Training)) across a health service reduced the proportion of term babies born with Apgar score <7 at 5 min (<75mins). DesignStepped-wedge cluster randomised controlled trial. SettingTwelve randomised maternity units with ≥900 births/year in Scotland. Three additional units were included in a supplementary analysis to assess the effect across Scotland. The intervention...
1h
BackgroundMany maternal and perinatal deaths in low-resource settings are preventable. Inadequate access to timely, quality care in maternity facilities drives poor outcomes, especially where women deliver at home with traditional birth attendants (TBA). Yet few solutions exist to support TBA-initiated referrals or address reasons patients frequently refuse facility care, such as disrespectful and abusive treatment. We hypothesised that deploying accompaniers—obstetric care navigators (OCN)—trained...
1h
Contributors : Gunnar H Poplawski ; Riki Kawaguchi ; Giovanni Coppola ; Mark TuszynskiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusIn the present study, we identify transcriptional mechanisms associated with corticospinal regeneration. We took advantage of the ability of NPC grafts to support corticospinal regeneration, together with a genetic mouse model (Glt25d2-GFP-L10a mice, in which a bacterial artificial chromosome (BAC) codes for GFP-tagged polyribosomes...
1h
Contributors : Yajun Tang ; Dong WangSeries Type : Expression profiling by high throughput sequencingOrganism : Fragaria vescaWith the development of high throughput sequencing technologies, plenty of non-coding RNAs (ncRNAs) have been discovered to play important roles in diverse plant biological processes. Although these ncRNAs extensively exist in plant, their biological functions are still remained to characterize. To obtain a comprehensive understanding of long non-coding RNA (lncRNA) function...
1h
Contributors : Emam Mehdi ; Mallard Bonnie ; Juan F Medrano ; Angela CanovasSeries Type : Expression profiling by high throughput sequencingOrganism : Bos taurusThe proinflammatory response of Monocyte-Derived Macrophages is controlled by host genetics
2h
Contributors : Ju-Gyeong Kang ; Ji-Eun Lee ; Kai Ge ; Paul M HwangSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusThe physiological effects of the many germline mutations of TP53, encoding the tumor suppressor protein p53, are poorly understood. Here, we report generating a p53 R178C knockin mouse modeling the human TP53 R181C mutation that is notable for its prevalence and prior molecular...
2h
Contributors : Michael A Garland ; Mitra C Geier ; Sean M Bugel ; Cheryl L Dunham ; Joseph M Brown ; Susan C Tilton ; Robyn L TanguaySeries Type : Expression profiling by high throughput sequencingOrganism : Danio rerioPolycyclic aromatic hydrocarbons (PAHs) typically alter gene expression by acting through the aryl hydrocarbon receptor (AHR). In a previous phenotypic screen of over 120 PAHs, we identified four PAHs that induce an ectopic caudal fin (called X-fin) in larval zebrafish: benzo[k]fluoranthene...
2h
Contributors : Lauge H Mikkelsen ; Steffen HeegaardSeries Type : Expression profiling by arrayOrganism : Homo sapiensNanoString data on 12 conjunctival melanomas and mucosal melanomaThe objective was to compare conjunctival melanoma with other mucosal melanomas at the RNA level using NanoString expression analysis of FFPE material.
2h
GSE143943 Post-sepsis immunosuppression depends on NKT cell regulation of mTOR/IFN-gamma in NK cells
Contributors : Edy Y Kim ; Hadas Ner-Gaon ; Aidan M Cullen ; Jingyu Guo ; Jiyoung Choi ; Tomoyoshi Tamura ; Tal Shay ; Michael B BrennerSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusWe show a new immunosuppressive function for NKT cells, and the NKT cell-mTOR axis may be a therapeutic target to prevent post-sepsis immunosuppression
2h
Contributors : Kaitlyn Liu ; Gregory Brent ; Jainrong LiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTraumatic Brain Injury (TBI) is associated with disruption of cerebral blood flow leading to localized brain hypoxia. Thyroid hormone (TH) treatment, when administered shortly after injury, has been shown to promote neural protection in rodent TBI models. The mechanism of TH protection, however, is not established. We used mouse primary cortical neurons...
2h
Contributors : Shinji Tanaka ; Hidewaki NakagawaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensBackground: The tumor microenvironment can be classified into immunologically active “inflamed” tumors and inactive “non-inflamed” tumors based on the infiltration of cytotoxic immune cells. Previous studies on liver cancer have reported a superior prognosis for inflamed tumors compared to non-inflamed tumors. However, liver cancer is highly heterogeneous immunologically...
2h
Contributors : Alec R Nickolls ; Michelle M Lee ; David F Espinoza ; Marcin Szczot ; Ruby Lam ; Qi Wang ; Jeanette Beers ; Jizhong Zou ; Minh Q Nguyen ; Hans J Solinski ; Aisha A AlJanahi ; Kory R Johnson ; Michael E Ward ; Alexander T Chesler ; Carsten G BönnemannSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensIn vitro generation of human peripheral sensory neurons may provide a framework for novel drug screening platforms and disease models of touch and...
2h
Contributors : Jun Zhang ; Jie QiSeries Type : Non-coding RNA profiling by arrayOrganism : Rattus norvegicusUsing the highly sensitive miRNA array, we screened 220 microRNAs abundant in physiological left ventricular hypertrophy (LVH) and we explored the functions of these miRNAs in the cardiac tissue by Gene Ontology and Kyoto Encyclopedia of Genes annotation. miRNAs showed a high score in the pathway enriched in autophagy. Moreover, the expression levels of miR-26b-5p, miR-204-5p, and miR-497-3p...
2h
Contributors : Chiara Tordonato ; Matteo J Marzi ; Pier P Di Fiore ; Francesco NicassioSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensAlthough ectopic overexpression of miRNAs can influence mammary normal and cancer stem cells (SCs/CSCs), their physiological relevance remains uncertain. Here, we found that the miR-146 family is linked to SC identity, since: i) their expression is very high in SCs/CSCs from human/mouse primary mammary tissues; correlates with...
2h
Contributors : Erika Dalmo ; Patrik Johansson ; Maria Niklasson ; Ida Gustavsson ; Sven Nelander ; Bengt WestermarkSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensGlioblastoma multiforme (GBM) continues to have a dismal prognosis. Even though detailed information on the genetic aberrations in cell signaling and cell cycle checkpoint control is available, no effective targeted treatment has been developed. Despite the advanced molecular defects, glioblastoma...
2h
Contributors : Sandi Chiranjeevi ; Messie Charlie ; Antonio Ramos-Montoya ; Sérgio L Felisbino ; Davies Barry ; Pacey SimonSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPurpose: RNA sequence analysis identified active proliferation of T cells in the combination therapyMethods: Pten-null tumours were collected 4 hours after the last dose (18th day). Total RNA from tumor samples was isolated using the Allprep kit (Qiagen, Germany). The quantity of the RNA...
2h
Contributors : Qiangsong Tong ; Liduan ZhengSeries Type : Expression profiling by arrayOrganism : Homo sapiensNeuroblastoma (NB), a malignant embryonic tumor arising from primitive neural crest cells, accounts for more than 7% of malignancies and around 15% of cancer-related mortality in childhood. Better elucidating the mechanisms of tumorigenesis and aggressiveness is important for improving the therapeutic efficiencies of NB. Through mining public microarray and RNA sequencing datasets, we identified...
5h
Contributor : Liexiang HuangfuSeries Type : Expression profiling by high throughput sequencingOrganism : Oryza sativaMelatonin plays a potential role in multiple plant developmental processes and stress response. However, there are no reports regarding exogenous melatonin promoting rice seed germination under salinity and nor about the underlying molecular mechanisms at genome-wide. Here, we revealed that exogenous application of melatonin conferred roles in promoting rice seed germination under...
5h
Contributor : Christopher K GlassSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusThe lung is inhabited by resident alveolar and interstitial macrophages as well as monocytic cells that survey the lung. Each cell type plays distinct functional roles under homeostatic and inflammatory conditions, but mechanisms establishing their molecular identities and functional potential remain poorly understood....
8h
Series Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
8h
Contributors : Eniko Sajti ; Verena M Link ; Zhengyu Ouyang ; Nathanael J Spann ; Emma Westin ; Casey E Romanoski ; Gregory J Fonseca ; Lawrence S Prince ; Christopher K GlassSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe lung is inhabited by resident alveolar and interstitial macrophages as well as monocytic cells that survey the lung. Each cell type plays distinct functional roles under homeostatic and inflammatory conditions, but mechanisms establishing...
8h
Contributors : Eniko Sajti ; Verena M Link ; Zhengyu Ouyang ; Nathanael J Spann ; Emma Westin ; Casey E Romanoski ; Gregory J Fonseca ; Lawrence S Prince ; Christopher K GlassSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe lung is inhabited by resident alveolar and interstitial macrophages as well as monocytic cells that survey the lung. Each cell type plays distinct functional roles under homeostatic and inflammatory conditions, but mechanisms establishing...
8h
Contributor : Gottfrid SjödahlSeries Type : Expression profiling by arrayOrganism : Homo sapiensIt is unclear how urothelial carcinoma of the urinary bladder change, genetically and phenotypically, as patients progress from recurrent non muscle-invasive (NMI) to muscle-invasive (MI) disease or disease that require radical cystectomy. This data set contains gene expression data from 200 samples in a longitudinal study of such patients. Overlap with previously GEO-published cohorts from our lab is...
10h
Series Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencing ; Methylation profiling by high throughput sequencingOrganism : Danio rerioThis SuperSeries is composed of the SubSeries listed below.
11h
Contributors : Hyung Joo Lee ; Ting Wang ; Stephen L JohnsonSeries Type : Expression profiling by high throughput sequencingOrganism : Danio rerioBackground: Zebrafish can faithfully regenerate injured fins through the formation of a blastema, a mass of proliferative cells that can grow and develop into the lost body part. After amputation, various cell types contribute to blastema formation, where each cell type retains fate restriction and exclusively contributes to regeneration of its own lineage....
11h
Contributors : Hyung Joo Lee ; Ting Wang ; Stephen L JohnsonSeries Type : Methylation profiling by high throughput sequencingOrganism : Danio rerioBackground: Zebrafish can faithfully regenerate injured fins through the formation of a blastema, a mass of proliferative cells that can grow and develop into the lost body part. After amputation, various cell types contribute to blastema formation, where each cell type retains fate restriction and exclusively contributes to regeneration of its own lineage....
11h
Contributors : Hyung Joo Lee ; Ting Wang ; Stephen L JohnsonSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Danio rerioBackground: Zebrafish can faithfully regenerate injured fins through the formation of a blastema, a mass of proliferative cells that can grow and develop into the lost body part. After amputation, various cell types contribute to blastema formation, where each cell type retains fate restriction and exclusively contributes to regeneration...
11h
Series Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
12h
Contributors : Martin A White ; Elise Darmon ; Manuel A Lopez-Vernaza ; David LeachSeries Type : OtherOrganism : Escherichia coli K-12The effect of a site-specific DNA double-strand break on the abundance of E. coli chromosomal DNA during exponential growth was investigated by marker frequency analysis (MFA).
15h
Contributors : Leon N Schulte ; Marco Metzger ; Jörg VogelSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiens ; Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344Here, we introduce an intestinal tissue model to study human enteric infections. Our model comprises epithelial and endothelial layers, a primary intestinal collagen scaffold, and immune cells. We use Dual RNA-seq to chart the communication amongst several different cell types at the...
16h
Series Type : Non-coding RNA profiling by high throughput sequencingOrganism : Mus musculusIntercellular communication orchestrates effective immune responses against disease-causing agents. Extracellular vesicles (EVs) are potent mediators of cell-cell communication. EVs carry bioactive molecules, including microRNAs, which modulate gene expression and function in the recipient cell. Here, we show that formation of cognate primary T-B lymphocyte immune contacts promotes transfer to the B cell of...
17h
Contributors : L Fernandez-Messina ; A Rodríguez-Galán ; V G García de Yébenes ; C Gutiérrez-Vázquez ; S Tenreiro ; A R Ramiro ; F Sanchez-MadridSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Mus musculusT cell blasts were obtained from wild-type C57BL/6 mice. Six-day culture blast supernatants were ultracentrifuged for small EV obtention and small EV pellet obtained after from 100,000g ultracentrifugation and T cell blasts RNA were isolated for small RNA sequencing;...
17h
Contributors : L Fernandez-Messina ; A Rodríguez-Galán ; V G García de Yébenes ; C Gutiérrez-Vázquez ; S Tenreiro ; A R Ramiro ; F Sanchez-MadridSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Mus musculusIntercellular communication orchestrates effective immune responses against disease-causing agents. Extracellular vesicles (EVs) are potent mediators of cell-cell communication. EVs carry bioactive molecules, including microRNAs, which modulate gene expression...
17h
Contributors : Takuya Nakagawa ; Masaki Fukuyo ; Atsushi KanedaSeries Type : Methylation profiling by genome tiling arrayOrganism : Homo sapiensDNA methylation analysis in oropharyngeal squamous carcinoma (OPSCC) samples and oropharyngeal non-cancerous mucosa samples. Infinium HumanMethylation450 BeadChip was used to obtain DNA methylation profiles across 485,577 CpG sites. Total samples included 89 OPSCC samples and 5 non-cancerous mucosa samples.
23h
Series Type : Methylation profiling by genome tiling arrayOrganism : Homo sapiensThis SuperSeries is composed of the SubSeries listed below.
23h
Contributors : Takuya Nakagawa ; Masaki Fukuyo ; Atsushi KanedaSeries Type : Methylation profiling by genome tiling arrayOrganism : Homo sapiensDNA methylation analysis in oropharyngeal squamous carcinoma (OPSCC) samples and oropharyngeal non-cancerous mucosa samples. Infinium MethylationEPIC BeadChip Kit was used to obtain DNA methylation profiles across more than 850,000 CpG sites. Total samples included 89 OPSCC samples and 5 non-cancerous mucosa samples.
23h
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